FlyBase Allele Report: Hsap\SNCA[UAS.cKa]

General Information

Symbol

Hsap\SNCA^UAS.cKa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Karpinar

FlyBase ID

FBal0269385

Feature type

allele

Associated gene

Hsap\SNCA

Associated Insertion(s)

Carried in Construct

M{UAS-αSyn.K}

Key Links

Transgenic product class

wild_type

(Karpinar et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Karpinar et al., 2009)

Mutagen

in vitro construct

(Karpinar et al., 2009)

Progenitor genotype

Carried in construct

M{UAS-αSyn.K}

Cytology

Description

UASt regulatory sequences drive expression of wild-type Hsap\SNCA.

(Karpinar et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\SNCA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Breda et al., 2015, Yin et al., 2014, Gajula Balija et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease

is ameliorated by Rab8^UASp.YFP

(Yin et al., 2014)

is ameliorated by Rab11^UAS.EGFP

(Breda et al., 2015)

is NOT ameliorated by Rab11^{S25N.UASp.YFP}

(Breda et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Expression of Hsap\SNCA^Scer\UAS.cKa affects the sleep behaviour of young flies prior to the stage when motor symptoms and neurotoxicity of the mutants can be detected.

(Gajula Balija et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Breda et al., 2015)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^C164

(Breda et al., 2015, Yin et al., 2014)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^ple.PF

(Breda et al., 2015, Yin et al., 2014)

abnormal locomotor rhythm, with Scer\GAL4^Ddc.PL

(Gajula Balija et al., 2011)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav.PU

(Yin et al., 2014)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ple.PF

(Yin et al., 2014)

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^elav-C155

(Breda et al., 2015)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Breda et al., 2015)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^elav-C155

(Breda et al., 2015)

short lived | adult stage, with Scer\GAL4^elav-C155

(Breda et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 results in an increase in the average amplitude of the evoked excitatory junction potentials (eEJPs) as well as the quantal content at neuromuscular junctions of third instar larvae and the frequency distribution of miniature excitatory junction potential (mEJP) amplitude sizes is significantly different compared to controls, although the mean mEJP amplitude is unchanged.

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 results in an increased size of synaptic vesicles and a shift in frequency distribution toward larger vesicles in both type 1b and 1s of neuromuscular junction boutons in third instar larvae compared to controls.

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF results in a decrease in the distance travelled per unit of time by third instar larvae in a crawling assay compared to controls.

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control Scer\GAL4^elav-C155 results in loss of dopaminergic neurons of the PPL1 cluster in the brain of adult flies.

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 results in defects in climbing behavior in adults of various ages (10, 20 and 30 day-old).

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 results in shortened lifespan of adult flies.

(Breda et al., 2015)

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF results in defects in locomotion and a significant decrease in the distance crawled by third instar larvae per unit of time compared to controls.

Ectopic expression of Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^ple.PF results in loss of dopaminergic neurons from the PPL1 and PPM3 clusters in adult flies compared to controls.

(Yin et al., 2014)

When compared to controls, expression of Hsap\SNCA^Scer\UAS.cKa in serotonergic and dopaminergic neurons, under the control of Scer\GAL4^Ddc.PL leads to a higher number of sleep episodes during the light phase but the average length of each sleep episode is not significantly affected. During dark phases, Hsap\SNCA^Scer\UAS.cKa expression leads to a reduction in the number of sleep episodes but an increase in the length of sleep episodes when compared to controlled flies. The overall level of activity is not affected.

Expression of Hsap\SNCA^Scer\UAS.cKa in serotonergic and dopaminergic neurons, under the control of Scer\GAL4^Ddc.PL alters circadian locomotor activity. Anticipation behaviour is maintained in young (1-3 days after eclosion) and old (30 days after eclosion) flies expressing Hsap\SNCA^Scer\UAS.cKa. In young flies, Hsap\SNCA^Scer\UAS.cKa expression causes a phasing out of locomotion activity at the light-dark transition. This behaviour is lost in older flies.

Flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^Ddc.PL display wild-type circardian periodicity.

(Gajula Balija et al., 2011)

The number of dopaminergic neurons in the dorsolateral (DL) and dorsomedial (DM) clusters in the brain of flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU is normal in both 2 and 29 day old adults.

25-30 day old flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU have normal climbing ability.

(Karpinar et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, enhanceable by Rab11^{S25N.UASp.YFP}, Scer\GAL4^elav-C155

(Breda et al., 2015)

Suppressed by

Statement

Reference

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^elav-C155

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has abnormal neurophysiology | third instar larval stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^elav-C155

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^C164 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^C164

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^ple.PF has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^ple.PF

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Scer\GAL4^elav-C155/Rab11^UAS.EGFP

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^elav-C155

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has short lived | adult stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^elav-C155

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^C164 has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Rab8^UASp.YFP, Scer\GAL4^C164

(Yin et al., 2014)

Hsap\SNCA^UAS.cKa, Scer\GAL4^ple.PF has abnormal locomotor behavior | third instar larval stage phenotype, suppressible by Rab8^UASp.YFP, Scer\GAL4^ple.PF

(Yin et al., 2014)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav.PU has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^elav.PU

(Yin et al., 2014)

Hsap\SNCA^UAS.cKa, Scer\GAL4^ple.PF has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^ple.PF

(Yin et al., 2014)

NOT suppressed by

Statement

Reference

Hsap\SNCA^UAS.cKa, Scer\GAL4^C164 has abnormal locomotor behavior | third instar larval stage phenotype, non-suppressible by Rab11^{S25N.UASp.YFP}, Scer\GAL4^C164

(Breda et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has synaptic vesicle | third instar larval stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^elav-C155

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^elav-C155

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Rab11^UAS.EGFP, Scer\GAL4^elav-C155

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa has NMJ bouton | third instar larval stage phenotype, suppressible by Scer\GAL4^elav-C155/Rab11^UAS.EGFP

(Breda et al., 2015)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav.PU has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by Scer\GAL4^elav.PU

(Yin et al., 2014)

Hsap\SNCA^UAS.cKa, Scer\GAL4^ple.PF has dopaminergic PPL1 neuron | adult stage phenotype, suppressible by Scer\GAL4^ple.PF

(Yin et al., 2014)

Hsap\SNCA^UAS.cKa, Scer\GAL4^elav.PU has dopaminergic PPM3 neuron | adult stage phenotype, suppressible | partially by Scer\GAL4^elav.PU

(Yin et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} suppresses increased eEJP amplitude, increased quantal content as well as increased synaptic vesicle size at boutons at the NMJs in third instar larvae expressing Hsap\SNCA^Scer\UAS.cKa driven by Scer\GAL4^elav-C155

The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} using either of the two drivers.

The defective crawling behavior (decrease in distance travelled per unit of time) characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^C164 cannot be suppressed by co-expression of Rab11^{S25N.Scer\UAS.P\T.T:Avic\GFP-YFP}.

The loss of dopaminergic PPL1 neurons in the adult brain characteristic for flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be reversed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP} in 30 day-old flies.

The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be suppressed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

The defects in climbing behavior seen in adult flies or various ages (10, 20 and 30 day-old) expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 are further worsened by co-expression of Rab11^{S25N.Scer\UAS.P\T.T:Avic\GFP-YFP}.

The shortened lifespan characteristic for adult flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155 can be reversed by co-expression of Rab11^{Scer\UAS.T:Avic\GFP-EGFP}.

(Breda et al., 2015)

The locomotion defects characteristic for third instar larvae expressing Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^C164 or Scer\GAL4^ple.PF can be suppressed by co-expression of Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP} : the decrease in the distance crawled by third instar larvae per unit of time is significantly ameliorated.

The reduction in the number of dopaminergic neurons from the PPL1 clusters characteristic for adult flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^ple.PF can be fully rescued by co-expression with Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP}. The loss of dopaminergic neurons from the PPM3 cluster seen in adult flies expressing Hsap\SNCA^Scer\UAS.cKa under the control of Scer\GAL4^elav.PU is partially restored by co-expression with Rab8^{Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Yin et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\SNCA^Scer\UAS.cKa

Hsap\SNCA^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Karpinar

Secondary FlyBase IDs

References (4)

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