Terminal filaments in ovaries from flies expressing tjNIG.10034R and NJF02959 under the control of Scer\GAL4tj-NP1624 are considerably shorter than those from flies expressing only tjNIG.10034R, although on average are still significantly longer than those in controls. Ovaries from flies expressing tjNIG.10034R and NJF02959 have a significant decrease in the number of terminal filaments, and some terminal filaments are unusually short with fewer than 7 cells.
Expression of both Nint.U.Scer\UAS and tjNIG.10034R under the control of Scer\GAL4tj-NP1624 leads to severe ovary defects. Most ovaries are extremely small, not connected to an oviduct and often attached to the gut, and consist largely of terminal filaments and a few germline cells embedded in fat body. The epithelial sheaths are missing and terminal filaments are located side-by-side. In the most severe cases, these ovaries consist only of terminal filaments. Terminal filaments in these flies are on average longer than those in flies expressing only Nint.U.Scer\UAS, but slightly shorter than those in flies expressing only tjNIG.10034R.