FlyBase Allele Report: Dmel\tj[NIG.10034R]

General Information

Symbol

Dmel\tj^NIG.10034R

Species

D. melanogaster

Name

FlyBase ID

FBal0270298

Feature type

allele

Associated gene

Dmel\tj

Associated Insertion(s)

Carried in Construct

P{NIG.10034R}

Also Known As

UAS-tj^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.10034R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-10034R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Df(2L)E55, Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

abnormal developmental rate, with Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

abnormal size | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

Phenotype Manifest In

border follicle cell, with Df(2L)E55, Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

border follicle cell, with Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

germarium cap cell | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

ovary | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

terminal filament | adult stage, with Scer\GAL4^NP1624

(Panchal et al., 2017)

Detailed Description

Statement

Reference

Expression of tj^NIG.10034R under the control of Scer\GAL4^tj-NP1624 leads to ovary defects, with abnormally long terminal filaments and absence of cap cells, as compared to controls.

(Panchal et al., 2017)

Compared with wild-type, expression of tj^NIG.10034R under the control of Scer\GAL4^slbo.2.6 delays border cell cluster migration by several hours in the ovary. Df(2L)E55/+ slightly enhances the migration defects in response to tj^NIG.10034R.

(Gunawan et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^slbo.2.6, tj^NIG.10034R has abnormal developmental rate phenotype, suppressible by slbo^UAS.cRa/Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

Scer\GAL4^slbo.2.6, tj^NIG.10034R has abnormal developmental rate phenotype, suppressible by shg^UASp.cSa/Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

Enhancer of

Statement

Reference

tj^NIG.10034R/Scer\GAL4^slbo.2.6 is an enhancer of abnormal developmental rate phenotype of slbo⁰¹³¹⁰

(Gunawan et al., 2013)

Other

Statement

Reference

N^JF02959, Scer\GAL4^NP1624, tj^NIG.10034R has abnormal size | adult stage phenotype

(Panchal et al., 2017)

N^CA.UAS.cUa, Scer\GAL4^NP1624, tj^NIG.10034R has abnormal size | adult stage phenotype

(Panchal et al., 2017)

N^CA.UAS.cUa, Scer\GAL4^NP1624, tj^NIG.10034R has decreased cell number | adult stage phenotype

(Panchal et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^slbo.2.6, tj^NIG.10034R has border follicle cell phenotype, suppressible by slbo^UAS.cRa/Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

Scer\GAL4^slbo.2.6, tj^NIG.10034R has border follicle cell phenotype, suppressible by shg^UASp.cSa/Scer\GAL4^slbo.2.6

(Gunawan et al., 2013)

Enhancer of

Statement

Reference

tj^NIG.10034R/Scer\GAL4^slbo.2.6 is an enhancer of border follicle cell phenotype of Scer\GAL4^slbo.2.6, slbo⁰¹³¹⁰

(Gunawan et al., 2013)

Other

Statement

Reference

N^JF02959, Scer\GAL4^NP1624, tj^NIG.10034R has ovary | adult stage phenotype

(Panchal et al., 2017)

N^JF02959, Scer\GAL4^NP1624, tj^NIG.10034R has terminal filament | adult stage phenotype

(Panchal et al., 2017)

N^CA.UAS.cUa, Scer\GAL4^NP1624, tj^NIG.10034R has ovary | adult stage phenotype

(Panchal et al., 2017)

N^CA.UAS.cUa, Scer\GAL4^NP1624, tj^NIG.10034R has terminal filament | adult stage phenotype

(Panchal et al., 2017)

N^CA.UAS.cUa, Scer\GAL4^NP1624, tj^NIG.10034R has germline cell | adult stage phenotype

(Panchal et al., 2017)

N^CA.UAS.cUa, Scer\GAL4^NP1624, tj^NIG.10034R has ovarian sheath | adult stage phenotype

(Panchal et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Terminal filaments in ovaries from flies expressing tj^NIG.10034R and N^JF02959 under the control of Scer\GAL4^tj-NP1624 are considerably shorter than those from flies expressing only tj^NIG.10034R, although on average are still significantly longer than those in controls. Ovaries from flies expressing tj^NIG.10034R and N^JF02959 have a significant decrease in the number of terminal filaments, and some terminal filaments are unusually short with fewer than 7 cells.

Expression of both N^{int.U.Scer\UAS} and tj^NIG.10034R under the control of Scer\GAL4^tj-NP1624 leads to severe ovary defects. Most ovaries are extremely small, not connected to an oviduct and often attached to the gut, and consist largely of terminal filaments and a few germline cells embedded in fat body. The epithelial sheaths are missing and terminal filaments are located side-by-side. In the most severe cases, these ovaries consist only of terminal filaments. Terminal filaments in these flies are on average longer than those in flies expressing only N^{int.U.Scer\UAS}, but slightly shorter than those in flies expressing only tj^NIG.10034R.

(Panchal et al., 2017)

Exogenous shg^{Scer\UAS.P\T.cSa} expression fully suppresses the moderate migration delay caused by Scer\GAL4^slbo.2.6>tj^NIG.10034R.

(Gunawan et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

10034R-1

P{NIG.10034R}1

10034R-2

P{NIG.10034R}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tj^NIG.10034R

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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