FlyBase Allele Report: Dmel\shg[UASp.cSa]

General Information

Symbol

Dmel\shg^UASp.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0294018

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{UASp-shg.S}

Also Known As

UAS-shg

Key Links

Transgenic product class

cDNA

(Sarpal et al., 2012)

wild_type

(Sarpal et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sarpal et al., 2012)

wild_type

(Sarpal et al., 2012)

Mutagen

in vitro construct

(Sarpal et al., 2012)

Progenitor genotype

Carried in construct

P{UASp-shg.S}

Cytology

Description

UASp regulatory sequences drive expression of shg cDNA.

(Sarpal et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

shg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

shg^UASp.cSa/Scer\GAL4^slbo.2.6 is a suppressor of abnormal developmental rate phenotype of Scer\GAL4^slbo.2.6, tj^NIG.10034R

(Gunawan et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, shg^UASp.cSa is a non-suppressor of abnormal cell migration | somatic clone | adult stage phenotype of α-Cat¹

(Desai et al., 2013)

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, shg^UASp.cSa is a non-suppressor of lethal - all die during embryonic stage phenotype of α-Cat¹

(Desai et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

shg^UASp.cSa/Scer\GAL4^slbo.2.6 is a suppressor of border follicle cell phenotype of Scer\GAL4^slbo.2.6, tj^NIG.10034R

(Gunawan et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, shg^UASp.cSa is a non-suppressor of follicle cell | somatic clone phenotype of α-Cat¹

(Desai et al., 2013)

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, shg^UASp.cSa is a non-suppressor of border follicle cell | somatic clone phenotype of α-Cat¹

(Desai et al., 2013)

Scer\GAL4^Act.PU, Scer\GAL4^da.G32, shg^UASp.cSa is a non-suppressor of embryonic head phenotype of α-Cat¹

(Desai et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU does not suppress the phenotypes seen in α-Cat¹ mutant follicle cell clones (generated through recombination of the α-Cat^Ubi.PS rescue construct). The border cell migration defects are also not rescued.

Ubiquitous expression of shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the embryonic head defects and lethality seen in α-Cat¹ mutant embryos.

(Desai et al., 2013)

Exogenous shg^{Scer\UAS.P\T.cSa} expression fully suppresses the moderate migration delay caused by Scer\GAL4^slbo.2.6>tj^NIG.10034R.

(Gunawan et al., 2013)

Expression of shg^{Scer\UAS.P\T.cSa} under the control of Scer\GAL4^da.G32 and Scer\GAL4^Act.PU fails to rescue the embryonic lethality seen in α-Cat¹ mutants.

Expressing shg^{Scer\UAS.P\T.cSa} does not allow α-Cat¹ mutant clones (generated through recombination of the α-Cat^Ubi.PS rescue construct) to develop in third instar larval imaginal discs. Mutant clone cells generated in the follicle epithelium lose cell contacts and border cells fail to migrate.

(Sarpal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

65589

w¹¹¹⁸; P{UASp-shg.S}attP2

65579

w¹¹¹⁸; P{tubP-GAL80}LL10, P{Ubi-α-Cat.S}4A P{neoFRT}40A; P{Act-GAL4.U}3, P{UASp-shg.S}attP2 α-Cat¹/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

shg^{Scer\UAS.P\T.cSa}

shg^UASp.cSa

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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