FlyBase Allele Report: Dmel\hoka[NIG.13704R]

General Information

Symbol

Dmel\hoka^NIG.13704R

Species

D. melanogaster

Name

FlyBase ID

FBal0271614

Feature type

allele

Associated gene

Dmel\hoka

Associated Insertion(s)

Carried in Construct

P{NIG.13704R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.13704R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-13704R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP0001

(Izumi et al., 2021)

adult midgut enterocyte | increased number, with Scer\GAL4^NP0001

(Izumi et al., 2021)

adult midgut epithelium, with Scer\GAL4^NP0001

(Izumi et al., 2021)

adult posterior midgut, with Scer\GAL4^NP0001

(Izumi et al., 2021)

intestinal stem cell | adult stage | increased number, with Scer\GAL4^NP0001

(Izumi et al., 2021)

Detailed Description

Statement

Reference

Adulthood-only expression of hoka^NIG.13704R under the control of Scer\GAL4^NP0001 leads to midgut defects: progressive barrier defects (in Smurf assays), increased number and increased proliferation of intestinal stem cells; increased diameter of posterior part of the midgut; enterocytes accumulate in the midgut lumen; enterocytes exhibit a variety of aberrant appearances, implying a polarity defect.

Adulthood-only expression under the control of Scer\GAL4^esg.PU results in an increased mitotic index in the adult midgut and enterocyte accumulation in the midgut lumen.

(Izumi et al., 2021)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP0001, hoka^NIG.13704R has increased occurrence of cell division | adult stage phenotype, suppressible | partially by Scer\GAL4^NP0001/aPKC^HMS01411

(Izumi et al., 2021)

Scer\GAL4^NP0001, hoka^NIG.13704R has increased occurrence of cell division | adult stage phenotype, suppressible | partially by yki^JF03119/Scer\GAL4^NP0001