FlyBase Allele Report: Dmel\His1[NIG.31617R]

General Information

Symbol

Dmel\His1^NIG.31617R

Species

D. melanogaster

Name

FlyBase ID

FBal0273500

Feature type

allele

Associated gene

His1

Associated Insertion(s)

Carried in Construct

P{NIG.31617R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.31617R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-31617R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

increased cell death | third instar larval stage, with Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

visible | adult stage, with Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Phenotype Manifest In

wing, with Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

wing disc | third instar larval stage, with Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Detailed Description

Statement

Reference

Expression of His1:CG31617^NIG.31617R under the control of Scer\GAL4^nub.PU leads to extensive apoptosis (assayed by activated Casp3 staining) in the the pouch region of third instar larval wing discs and to severely underdeveloped small and deformed adult wings.

(Bayona-Feliu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, enhanceable by p53^H159N.UAS.Ex, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, enhanceable by p53^H159N.UAS.Ex, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has increased cell death | third instar larval stage phenotype, enhanceable by p53^H159N.UAS.Ex, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Suppressed by

Statement

Reference

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by lok^KK100813, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible | partially by lok^KK100813, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by p53^GD4483, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible | partially by p53^GD4483, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by puc^UAS.cMa, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible | partially by puc^UAS.cMa, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by bsk^KK108156, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible | partially by bsk^KK108156, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has increased cell death | third instar larval stage phenotype, suppressible by mei-41^KK101792, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has increased cell death | third instar larval stage phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has increased cell death | third instar larval stage phenotype, suppressible by bsk^KK108156, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by Hsap\RNASEH1^UAS.cBa, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by mei-41^KK101792, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible | partially by mei-41^KK101792, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by tefu^GD11950, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible | partially by tefu^GD11950, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has visible | adult stage phenotype, suppressible | partially by grp^GD4189, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has abnormal size | adult stage phenotype, suppressible | partially by grp^GD4189, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

NOT suppressed by

Statement

Reference

His1^NIG.31617R, Scer\GAL4^nub.PU has increased cell death | third instar larval stage phenotype, non-suppressible by p53^GD4483, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

His1^NIG.31617R, Scer\GAL4^nub.PU has wing disc | third instar larval stage phenotype, enhanceable by p53^H159N.UAS.Ex, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Suppressed by

Statement

Reference

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by mei-41^KK101792, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by tefu^GD11950, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by grp^GD4189, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by lok^KK100813, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by p53^GD4483, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by puc^UAS.cMa, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by bsk^KK108156, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing disc | third instar larval stage phenotype, suppressible by mei-41^KK101792, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, suppressible | partially by Hsap\RNASEH1^UAS.cBa, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing disc | third instar larval stage phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing disc | third instar larval stage phenotype, suppressible by bsk^KK108156, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

NOT suppressed by

Statement

Reference

His1^NIG.31617R, Scer\GAL4^nub.PU has wing phenotype, non-suppressible by p53^H159N.UAS.Ex, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

His1^NIG.31617R, Scer\GAL4^nub.PU has wing disc | third instar larval stage phenotype, non-suppressible by p53^GD4483, Scer\GAL4^nub.PU

(Bayona-Feliu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The extensive cell death in the pouch region of wing discs in third instar larvae as well as the small and deformed wings in adults expressing His1:CG31617^NIG.31617R under the control of Scer\GAL4^nub.PU are significantly suppressed by co-expression of any of the following mei-41^KK101792, puc^Scer\UAS.cMa or bsk^KK108156 but not p53^GD4483 whereas co-expression of p53^{H159N.Scer\UAS.Ex} enhances both the larval and adult phenotypes. The adult wing defects can also be partially rescued by co-expression of tefu^GD11950, grp^GD4189 or lok^KK100813.

(Bayona-Feliu et al., 2017)

Xenogenetic Interactions

Statement

Reference

The small and deformed wings characteristic for flies expressing His1:CG31617^NIG.31617R under the control of Scer\GAL4^nub.PU can be partially rescued by co-expression of Hsap\RNASEH1^Scer\UAS.cBa.

(Bayona-Feliu et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

31617R-2

P{NIG.31617R}2

31617R-3

P{NIG.31617R}3