FlyBase Allele Report: Dmel\Sarm[UAS.cOa]

General Information

Symbol

Dmel\Sarm^UAS.cOa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Osterloh

FlyBase ID

FBal0277680

Feature type

allele

Associated gene

Dmel\Sarm

Associated Insertion(s)

Carried in Construct

P{UAS-Sarm.O}

Also Known As

UAS-dsarm

Key Links

Transgenic product class

cDNA

(Osterloh et al., 2012)

wild_type

(Osterloh et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Osterloh et al., 2012)

wild_type

(Osterloh et al., 2012)

Mutagen

in vitro construct

(Osterloh et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Sarm.O}

Cytology

Description

UAS regulatory sequences drive expression of a full-length Ect4 cDNA.

(Osterloh et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sarm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Osterloh et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR58E02

(Singh et al., 2025)

abnormal neuroanatomy | adult stage, with Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

decreased cell number | adult stage, with Scer\GAL4^GMR58E02

(Singh et al., 2025)

decreased cell number | embryonic stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

Phenotype Manifest In

adult dopaminergic PAM neuron | decreased number, with Scer\GAL4^GMR58E02

(Singh et al., 2025)

adult mechanosensory neuron, with Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

axon | adult stage, with Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

larval central nervous system | embryonic stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

neuron | embryonic stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

Detailed Description

Statement

Reference

Expression of Ect4^Scer\UAS.cOa under the control of Scer\GAL4^elav.PU results in a loss of eve-positive neurons from the EL+U/CQ class (whereas numbers in of the aCC, pCC ans RP2 neurons are not significantly different from the controls) in the embryonic central nervous system as compared to driver-only controls.

(Foldi et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Wnk^kd.UAS, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Suppressed by

Statement

Reference

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Wnk^UAS.cIa, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

NOT suppressed by

Statement

Reference

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Wnk^kd.UAS, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has axon | adult stage phenotype, non-enhanceable by Wnk^kd.UAS, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has adult mechanosensory neuron phenotype, non-enhanceable by Wnk^kd.UAS, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Suppressed by

Statement

Reference

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has axon | adult stage phenotype, suppressible | partially by Wnk^UAS.cIa, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has adult mechanosensory neuron phenotype, suppressible | partially by Wnk^UAS.cIa, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

NOT suppressed by

Statement

Reference

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has axon | adult stage phenotype, non-suppressible by Wnk^kd.UAS, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Sarm^UAS.cOa, Scer\GAL4^pnr-MD237 has adult mechanosensory neuron phenotype, non-suppressible by Wnk^kd.UAS, Scer\GAL4^pnr-MD237

(Izadifar et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Or22a.7.717/Sarm^UAS.cOa rescues Sarm⁸⁹⁶

(Osterloh et al., 2012)

Partially rescues

Scer\GAL4^VGlut1-OK371/Sarm^UAS.cOa partially rescues Sarm⁸⁹⁶

(Neukomm et al., 2017)

Comments

The axon death defect (severed sensory neuron axons in the adult wing persist after an axotomy, instead of being cleared away) observed in flies carrying Ect4⁸⁹⁶ neuronal mutant clones can be partially rescued by Scer\GAL4^VGlut-OK371-driven expression of Ect4^Scer\UAS.cOa in the mutant clones.

(Neukomm et al., 2017)

Expression of Ect4^Scer\UAS.cOa under the control of Scer\GAL4^Or22a.7.717 reverts the suppression of axonal degeneration after axon severing which is seen in Ect4⁸⁹⁶ mutant neurons.

(Osterloh et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ect4^Scer\UAS.cOa

Ect4^UAS.cOa

Sarm^UAS.cOa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Osterloh

Secondary FlyBase IDs

References (7)

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