FlyBase Allele Report: Dmel\Atf3[CH321-51N24.EGFP]

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Allele: Dmel\Atf3^{CH321-51N24.EGFP}

General Information

Symbol

Dmel\Atf3^{CH321-51N24.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0277813

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

M{Atf3-EGFP.CH321-51N24}

Key Links

Transgenic product class

(Rynes et al., 2012)

(Rynes et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Rynes et al., 2012)

(Rynes et al., 2012)

Mutagen

in vitro construct

(Rynes et al., 2012)

Progenitor genotype

Carried in construct

M{Atf3-EGFP.CH321-51N24}

Cytology

Description

Contains the CH321-51N24 BAC clone which includes Atf3. The Atf3 coding region has been tagged at the C-terminal end with EGFP.

(Rynes et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Tagged with

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

• nucleus

(Rynes et al., 2012)

larval ventral nerve cord

• nucleus

(Rynes et al., 2012)

• nucleus

(Rynes et al., 2012)

corpus cardiacum

• nucleus

(Rynes et al., 2012)

• nucleus

(Rynes et al., 2012)

eye-antennal disc

• nucleus

(Rynes et al., 2012)

peripodial epithelium of wing disc

• nucleus

(Rynes et al., 2012)

embryonic/larval midgut

• nucleus

(Rynes et al., 2012)

embryonic/larval hindgut

• nucleus

(Rynes et al., 2012)

embryonic/larval proventriculus

• nucleus

(Rynes et al., 2012)

• nucleus

(Rynes et al., 2012)

embryonic/larval fat body

• nucleus

(Rynes et al., 2012)

embryonic/larval salivary gland

• nucleus

(Rynes et al., 2012)

• nucleus

(Rynes et al., 2012)

embryonic/larval Malpighian tubule

• nucleus

(Rynes et al., 2012)

Additional Information

Statement

Reference

Atf3 protein, as assayed by use of a GFP-tagged transgenic construct Atf3^{CH321-51N24.T:AvicGFP-EGFP}, is localized to the nucleus in tissues central to metabolic and immune homeostasis, including the gut, salivary glands, fat body, ring gland, and CNS.

(Rynes et al., 2012)

Marker for

Reflects expression of

(Rynes et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Atf3^{CH321-51N24.EGFP} rescues Atf3⁷⁶

(Rynes et al., 2012)

Comments

One copy of Atf3^{CH321-51N24.T:Avic\GFP-EGFP} completely rescues the developmental delay and adult eclosion of Atf3⁷⁶/Y males and permits the survival of homozygous Atf3⁷⁶ females.

(Rynes et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Atf3^{CH321-51N24.EGFP}

Atf3^{CH321-51N24.T:Avic\GFP-EGFP}

atf3^gBAC

(Donohoe et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections