Contains a genomic fragment centred on Rab39 in which part of the Rab39 open reading frame (extending from the ATG to the end of the ATG-containing exon) has been replaced with the GAL4 driver coding region and a loxP cassette containing Disc\RFP3xP3.PC and the kanamycin resistance gene.
Comment: 30-40h APF
Comment: 30-40h APF
Comment: 30-40h APF
ScerGAL4Rab39.ATG drives expression in the larval brain, as well as in the eye, wing and leg imaginal discs. In the pupal and adult brain, expression is widespread.
The PBac{I-SceI(FRT.Rab39-GAL4.ATG(loxP.3xP3-RFP))} vector can be used both as a driver expressing Scer\GAL4 under the control of the Rab39 regulatory sequences present in the construct and also as a donor construct for ends-out homologous recombination at the endogenous Rab39 locus.