FlyBase Allele Report: Dmel\Ras85D[R68Q]

General Information

Symbol

Dmel\Ras85D^R68Q

Species

D. melanogaster

Name

FlyBase ID

FBal0285348

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

Also Known As

ras1^R68Q

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

alkylating agent

Nature of the Allele

Allele class

Mutagen

alkylating agent

(Gafuik and Steller, 2011)

Progenitor genotype

Cytology

Description

Amino acid replacement: R68Q.

(Gafuik and Steller, 2011)

Nucleotide substitution: G641A.

(Gafuik and Steller, 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:9,511,933..9,511,933 [-]

Nucleotide change:

G9511933A

Reported nucleotide change:

G641A

Amino acid change:

R68Q | Ras85D-PA

Reported amino acid change:

R68Q

(Gafuik and Steller, 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death

(Gafuik and Steller, 2011)

Phenotype Manifest In

eye

(Gafuik and Steller, 2011)

midline glial cell

(Gafuik and Steller, 2011)

ommatidium

(Gafuik and Steller, 2011)

photoreceptor cell R7

(Gafuik and Steller, 2011)

posterior crossvein

(Gafuik and Steller, 2011)

wing

(Gafuik and Steller, 2011)

wing vein | ectopic

(Gafuik and Steller, 2011)

wing vein L4

(Gafuik and Steller, 2011)

wing vein L5

(Gafuik and Steller, 2011)

Detailed Description

Statement

Reference

Flies mutant for Ras85D^R68Q exhibit a number of developmental defects that are characteristic of abnormally elevated RTK/RAS/MAPK signaling, including enhanced resistance to apoptosis, supernumerary R7 cells in the eye and ectopic wing vein formation.

Ras85D^R68Q mutants have a reduced basal level of GTPase activity.

Ras85D^R68Q mutants exhibit a statistically significant increase in the number of midline glia cells at stage 17 of embryogenesis, compared to controls.

Ras85D^R68Q mutants exhibit supernumerary R7 cells in the developing ommatidia. Occasionally, ommatidia are found without outer photoreceptor cells, a phenotypic consequence of elevated MAPK signaling.

Ras85D^R68Q flies show abnormalities in adult wing tissues. Homozygous Ras85D^R68Q flies have an extra longitudinal 'veinlet' branching off the posterior crossvein. Additionally, an ectopic longitudinal vein is seen beneath the posterior crossvein and there is an ectopic crossvein found between the L4 and L5 wing veins near the hinge.

(Gafuik and Steller, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ras85D^R68Q has decreased cell death phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

NOT suppressed by

Statement

Reference

Ras85D^R68Q has decreased cell death phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has decreased cell death phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Suppressor of

Statement

Reference

Ras85D^R68Q is a suppressor of increased cell death phenotype of hid^Ala3.GMR

(Gafuik and Steller, 2011)

Ras85D^R68Q is a suppressor of increased cell death phenotype of hid^GMR.PG

(Gafuik and Steller, 2011)

NOT Suppressor of

Statement

Reference

Ras85D^R68Q is a non-suppressor of increased cell death phenotype of hid^Ala5.GMR

(Gafuik and Steller, 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^R68Q has photoreceptor cell R7 phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein | ectopic phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has eye phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has midline glial cell phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has ommatidium phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has posterior crossvein phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has crossvein phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein L4 phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein L5 phenotype, suppressible by rl^10a

(Gafuik and Steller, 2011)

NOT suppressed by

Statement

Reference

Ras85D^R68Q has photoreceptor cell R7 phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein | ectopic phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has photoreceptor cell R7 phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein | ectopic phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has eye phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has midline glial cell phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has ommatidium phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has posterior crossvein phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has crossvein phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein L4 phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein L5 phenotype, non-suppressible by aos^Δ7

(Gafuik and Steller, 2011)

Ras85D^R68Q has eye phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has midline glial cell phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has ommatidium phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has posterior crossvein phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has crossvein phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein L4 phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Ras85D^R68Q has wing vein L5 phenotype, non-suppressible by Egfr^f2

(Gafuik and Steller, 2011)

Suppressor of

Statement

Reference

Ras85D^R68Q is a suppressor | partially of hemocyte | larval stage phenotype of Scer\GAL4^Hml.PG, hid^UAS.cZa

(Gafuik and Steller, 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Ras85D^R68Q suppresses W^GMR.PG induced cell death in a dosage-dependent manner.

Ras85D^R68Q suppresses W^Ala3.GMR (which lacks 3 of the 5 predicted MAPK phosphorylation sites) induced cell death.

Ras85D^R68Q fails to suppress W^Ala5.GMR (which lacks all of the 5 predicted MAPK phosphorylation sites) induced cell death.

Ras85D^R68Q very mildly suppresses grim^GMR.PC and rpr^GMR.PW induced cell death although this is suspected to be due to suppression of endogenous W activity and not grim or rpr activity.

A Ras85D^R68Q background can partially suppress the hemocyte death seen upon expression of W^Scer\UAS.cZa under the control of Scer\GAL4^Hml.PG. Hemocytes are clearly visible anteriorly in the lymph glands of 3rd instar larvae. Circulating hemocytes, however, appear to remain susceptible to W-induced cell death and are missing, even in the presence of two copies of Ras85D^R68Q.

An Egfr^f2 background does not affect the developmental and apoptotic defects found in Ras85D^R68Q mutants.

A aos^Δ7 background does not affect the developmental and apoptotic defects found in Ras85D^R68Q mutants.

A rl^10a background strongly ameliorates the developmental and apoptotic defects found in Ras85D^R68Q mutants.

(Gafuik and Steller, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ras85D^R68Q is rescued by Ras85D^N17.sev

(Gafuik and Steller, 2011)

Comments

Ras85D^N17.sev expression in the eye abrogates the suppressive effects of Ras85D^R68Q on W^GMR.PG-induced apoptosis.

(Gafuik and Steller, 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ras1^R68Q

(Fenckova et al., 2019)

Ras85D^R68Q

Su(21-3s)

(Gafuik and Steller, 2011)

ras1^R68Q

(Gafuik and Steller, 2011)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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