FlyBase Allele Report: Dmel\plum[ΔCyt.UAS.Tag:FLAG]

General Information

Symbol

Dmel\plum^{ΔCyt.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0288235

Feature type

allele

Associated gene

Dmel\plum

Associated Insertion(s)

Carried in Construct

P{UAS-plum.ΔCyt.Flag}

Also Known As

UAS-plum^ΔCyt

Key Links

Transgenic product class

inframe_deletion

(Yu et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Yu et al., 2013)

Mutagen

in vitro construct

(Yu et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-plum.ΔCyt.Flag}

Cytology

Description

UAS regulatory sequences drive expression of plum lacking the cytoplasmic domain (deletion extends from amino acid residue937 to the C-terminal end of the protein). The protein is tagged with Tag:FLAG.

(Yu et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

plum

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Marmor-Kollet et al., 2019)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^repo.PU

(Yu et al., 2013)

abnormal size | adult stage, with Scer\GAL4^ey-OK107

(Marmor-Kollet et al., 2019)

Phenotype Manifest In

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Marmor-Kollet et al., 2019)

gamma Kenyon cell, with Scer\GAL4^repo.PU

(Yu et al., 2013)

Detailed Description

Statement

Reference

Expressing plum^{ΔCyt.UAS.Tag:FLAG} under the control of Scer\GAL4^ey-OK107 induces adult mushroom body β-lobe retraction in almost half of cases.

(Marmor-Kollet et al., 2019)

Expression of plum^{ΔCyt.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^repo.PU results in a dose-dependent axon pruning defect in the mushroom body γ neurons.

(Yu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^repo.PU, plum^{ΔCyt.UAS.Tag:FLAG} has abnormal neuroanatomy | pupal stage phenotype, suppressible | partially by Scer\GAL4^repo.PU/myo^UAS.cAa

(Yu et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^repo.PU/plum^{ΔCyt.UAS.Tag:FLAG} is a suppressor | partially of lethal phenotype of Scer\GAL4^repo.PU, myo^UAS.cAa

(Yu et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^repo.PU, plum^{ΔCyt.UAS.Tag:FLAG} has gamma Kenyon cell phenotype, suppressible | partially by Scer\GAL4^repo.PU/myo^UAS.cAa

(Yu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality caused by expression of myo^Scer\UAS.cAa under the control of Scer\GAL4^repo.PU is partially suppressed by co-expression of plum^{ΔCyt.Scer\UAS.T:Zzzz\FLAG} : 31% of animals survive to adulthood. Co-expression of myo^Scer\UAS.cAa partially suppresses the axon pruning defect see in the mushroom body γ neurons of animals expressing plum^{ΔCyt.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^repo.PU.

(Yu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Tab2-201Y/plum^{ΔCyt.UAS.Tag:FLAG} rescues plum^Δ1

(Yu et al., 2013)

Scer\GAL4^elav.PU/plum^{ΔCyt.UAS.Tag:FLAG} rescues plum^Δ2

(Yu et al., 2013)

Fails to rescue

Scer\GAL4^GMR44E04/plum^{ΔCyt.UAS.Tag:FLAG} fails to rescue plum^Δ1

(Marmor-Kollet et al., 2019)

Comments

Expression of plum^{ΔCyt.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Tab2-201Y rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of plum^{ΔCyt.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU rescues the formation of ectopic connections at the neuromuscular junction in plum^Δ2 larvae.

(Yu et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Plum^ΔCyt

(Yu et al., 2013)

plum^{ΔCyt.Scer\UAS.T:Zzzz\FLAG}

plum^{ΔCyt.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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