FlyBase Allele Report: Scer\GAL4[GMR44E04]

General Information

Symbol

Scer\GAL4^GMR44E04

Species

S. cerevisiae

Name

FlyBase ID

FBal0252595

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR44E04-GAL4}

Also Known As

R44E04, R44E04-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR44E04-GAL4}

Cytology

Description

A 2855 base pair fragment from the flanking non-coding or intronic region of EcR is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR44E04

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

alpha/beta Kenyon cell

(Bouzaiane et al., 2015)

adult brain | restricted

(Bouzaiane et al., 2015)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Blockade of all αβ Kenyon cells (by expressing the temperature sensitive shi^ts.UAS.cUa under the control of Scer\GAL4^GMR44E04 and shifted to the permissive 33[o]C) decreases olfactory medium term memory (1h) when the experiment is carried out in crowded conditions (groups of 32 flies - socially facilitated memory).

(Muria et al., 2021)

Inhibition (using shi^1.UAS at 33[o]C) of Scer\GAL4^GMR44E04 cells in adults significantly reduces 5 min or 30 min aversive olfactory memory, including its anaesthesia-resistant component, compared to controls; inhibition during testing significantly reduces medium term (3 h) aversive olfactory memory, but not its anaesthesia-resistant component, compared to controls, with no effect on long term (24 h after massed training) memory; inhibition does not affect olfactory acuity, compared to controls.

(Bouzaiane et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^GMR44E04/RhoGEF2^UAS.cUa is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of plum^Δ1

(Marmor-Kollet et al., 2019)

Scer\GAL4^GMR44E04/RhoGEF2^UAS.cUa is a suppressor | partially of abnormal size | adult stage phenotype of plum^Δ1