FlyBase Allele Report: Ecol\lexA[GMR50G08]

General Information

Symbol

Ecol\lexA^GMR50G08

Species

E. coli

Name

FlyBase ID

FBal0289698

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{GMR50G08-lexA}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2013.10.9)

Progenitor genotype

Carried in construct

P{GMR50G08-lexA}

Cytology

Description

A 1091 base pair fragment from the flanking non-coding or intronic region of VGlut is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR50G08

Encoded product / tool

lexA::p65

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | larval stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}

(MacNamee et al., 2016)

Phenotype Manifest In

embryonic/larval astrocyte-like glial cell | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}

(MacNamee et al., 2016)

embryonic/larval motor neuron | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}

(MacNamee et al., 2016)

larval RP2 motor neuron | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}

(MacNamee et al., 2016)

larval VL3/4 motor neuron | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}

(MacNamee et al., 2016)

Detailed Description

Statement

Reference

Optogenetic activation (using Crei\ChR2^{H134R.UAS.mCherry} with 470 nm (blue) light) of Ecol\lexA^GMR50G08 cells in larvae results in inhibitory postsynaptic currents in multiple types of motor neuron, including RP2 and RP3, and inward currents in astrocytes, which are not seen in unstimulated conditions.

(MacNamee et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Eaat1^KK100187, Ecol\lexA^GMR50G08, Scer\GAL4^alrm.PD has abnormal neurophysiology | larval stage | conditional phenotype

(MacNamee et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Ecol\lexA^GMR50G08 has embryonic/larval astrocyte-like glial cell | conditional phenotype, suppressible by Eaat1^KK100187/Scer\GAL4^alrm.PD

(MacNamee et al., 2016)

Other

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Eaat1^KK100187, Ecol\lexA^GMR50G08, Scer\GAL4^alrm.PD has larval RP2 motor neuron | conditional phenotype

(MacNamee et al., 2016)

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Eaat1^KK100187, Ecol\lexA^GMR50G08, Scer\GAL4^alrm.PD has embryonic/larval motor neuron | conditional phenotype

(MacNamee et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Eaat1^KK100187 under the control of Scer\GAL4^alrm.PD fully suppresses the inward currents in astrocytes and significantly alters the properties of the inhibitory postsynaptic currents in motor neurons, including RP2, that result from optogenetic activation (using Crei\ChR2^{H134R.UAS.mCherry} with 470 nm (blue) light) of Ecol\lexA^GMR50G08 cells in larvae.

(MacNamee et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

53494

w¹¹¹⁸; P{GMR50G08-lexA}attP40

Notes on Origin

Discoverer

Comments

Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

(Pfeiffer et al., 2013.10.9)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ecol\lexA^GMR50G08

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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