A 3200 base pair fragment from the flanking non-coding or intronic region of CG31760 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.lexAop.Citrine with 617 nm (red) light) of Ecol\lexAGMR72F11 cells in larvae results in an induction of nociceptive rolling behavior in the absence of nociceptive stimuli, unlike controls.
Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.lexAop.Citrine, Ecol\lexAGMR72F11 has abnormal pain response | larval stage | conditional phenotype, non-enhanceable by Ctet\tetXTNT-LC.UAS/Scer\GAL4GMR94B10
Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.lexAop.Citrine, Ecol\lexAGMR72F11 has abnormal pain response | larval stage | conditional phenotype, non-suppressible by Ctet\tetXTNT-LC.UAS/Scer\GAL4GMR94B10
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.