FB2026_02 , released June 18, 2026
Allele: Dmel\wgKO.FRT.wg.FRT.NRT-wg
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General Information
Symbol
Dmel\wgKO.FRT.wg.FRT.NRT-wg
Species
D. melanogaster
Name
FlyBase ID
FBal0297883
Feature type
allele
Associated gene
Dmel\wg
Associated Insertion(s)
TI{TI}wgKO.FRT.wg.FRT.NRT-wg
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Alexandre et al., 2014, Baena-Lopez et al., 2013)
ends-out gene targeting
(Baena-Lopez et al., 2013)
cre recombinase
(Baena-Lopez et al., 2013)
FLPase
(Baena-Lopez et al., 2013)
I-SceI
(Baena-Lopez et al., 2013)
Progenitor genotype
wgKO
(Alexandre et al., 2014)
Associated Insertion(s)
TI{TI}wgKO.FRT.wg.FRT.NRT-wg
Cytology
Description

Re-integration of DNA into the attP site present in wgKO. The reintegrated sequence is as follows: 1. an FRT cassette containing the full-length, wild-type wg cDNA sequence (including 135 bp of 5'UTR and 1200bp of 3'UTR sequence), 2. 'Nrt-wg' (a membrane-tethered, Tag:HA-tagged form of wg in which Tag:M(Nrt) acts as the membrane tethering sequence), and 3. a loxP cassette containing a Disc\RFPmCherry.3xP3 marker. Nrt-wg is not expressed due to the presence of the upstream FRT cassette. In addition, a recombined attP/B site is present 5' to the reintegrated sequence.

(Alexandre et al., 2014)

Part of the wg first exon (including the ATG site and all the coding sequence of this exon) has been deleted. The deleted sequence has been replaced with the following reintegrated sequence: 1. an FRT cassette containing the wild-type wg coding region, 2. coding sequence for a membrane-tethered, Tag:HA-tagged form of wg (Tag:M(Nrt) acts as the membrane tethering sequence), and 3. a loxP cassette containing the Disc\RFPmCherry.3xP3 marker. The membrane-tethered, Tag:HA-tagged form of wg is not expressed due to the presence of the upstream FRT cassette. A recombined attP/B site is present 5' to the FRT cassette.

(Baena-Lopez et al., 2013)
Allele components
Component
Use(s)
Also carries
FRT
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      "wg{KO; WT[Body]; NRT-Wg[Discs]}" larvae expressing 'NRT-wg' (a membrane-tethered form of wg) only in imaginal discs (and wild-type wg in other tissues) have been generated by expressing FLPase in the imaginal discs of homozygous wgKO;FRT.wg.FRT.NRT-wg larvae (converting wgKO;FRT.wg.FRT.NRT-wg to wgKO;-FRT.NRT-wg in the discs). These larvae develop at the same rate as controls and eclose at near normal frequency when co-cultured with control siblings. The adult flies are 23% smaller than controls and the wing margin lacks occasional bristles.

      (Alexandre et al., 2014)
      External Data
      Interactions
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      FRT Wg FRT NRT-Wg
      (Alexandre et al., 2014)
      wgKO.FRT.wg.FRT.NRT-wg
      wgKO;FRT.wg.FRT.NRT-HA-wg
      wgKO;FRT.wg.FRT.NRT-wg
      wg[KO; FRT.Wg.FRT.NRT-HA-Wg]
      (Baena-Lopez et al., 2013)
      wg[cNRT]
      (Yu et al., 2020)
      wg{KO;FRT.WgFRT.NRT-HA-Wg}
      (Alexandre et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)