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General Information
Symbol
Dmel\Fer2miRNA.UAS.5
Species
D. melanogaster
Name
FlyBase ID
FBal0299746
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-miR Fer2-5
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a mir-1 pre mRNA scaffold containing a short hairpin miRNA targeting Fer2 (5'-TCAAAGCGGATAGGGCTAATT-3').

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Fer2miRNA.Scer\UAS.5 under the control of Scer\GAL4Fer2.2359 has no effect on PAM dopaminergic neuron number until 35 days of age. However, at 49 days many flies have significantly fewer PAM neurons compared to controls. Late stage PAM neuron loss is also seen when Fer2miRNA.Scer\UAS.5 is expressed under the control of either Scer\GAL4GMR58E02 or Scer\GAL4Ddc.HL9. The PAL neurons are unaffected.

Restricting expression of Fer2miRNA.Scer\UAS.5 to the adult stages using Scer\GAL80 induces adult-onset progressive degeneration of PAM dopaminergic neurons without affecting PAL neurons.

Expression of Fer2miRNA.Scer\UAS.5 in PAM neurons under the control of Scer\GAL4Ddc.HL9 results in a significant decline in climbing ability after 49 days.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Phenotype Manifest In
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

The expression of Fer2miRNA.UAS.5 under the control of Scer\GAL4Fer2.2359 enhances the progressive decrease in the number of adult PAM neurons but does not affect the number of adult PAL cluster neurons observed in foxoΔ94 homozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Fer2miRNA.Scer\UAS.5
Fer2miRNA.UAS.5
Name Synonyms
Secondary FlyBase IDs
    References (2)