FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Fer2RNAi.UAS.5
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General Information
Symbol
Dmel\Fer2RNAi.UAS.5
Species
D. melanogaster
Name
FlyBase ID
FBal0299746
Feature type
allele
Associated gene
Dmel\Fer2
Associated Insertion(s)
Carried in Construct
P{UAS-Fer2.RNAi.5}
Also Known As
UAS-miR Fer2-5
Key Links
Transgenic product class
RNAi_reagent
(Bou Dib et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Bou Dib et al., 2014)
Mutagen
in vitro construct
(Bou Dib et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-Fer2.RNAi.5}
Cytology
Description

UAS regulatory sequences drive expression of a mir-1 pre mRNA scaffold containing a short hairpin miRNA targeting Fer2 (5'-TCAAAGCGGATAGGGCTAATT-3').

(Bou Dib et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Fer2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Parkinson's disease
      modeled by Fer2MB09480
      (Tas et al., 2018)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Fer2miRNA.Scer\UAS.5 under the control of Scer\GAL4Fer2.2359 has no effect on PAM dopaminergic neuron number until 35 days of age. However, at 49 days many flies have significantly fewer PAM neurons compared to controls. Late stage PAM neuron loss is also seen when Fer2miRNA.Scer\UAS.5 is expressed under the control of either Scer\GAL4GMR58E02 or Scer\GAL4Ddc.HL9. The PAL neurons are unaffected.

      Restricting expression of Fer2miRNA.Scer\UAS.5 to the adult stages using Scer\GAL80 induces adult-onset progressive degeneration of PAM dopaminergic neurons without affecting PAL neurons.

      Expression of Fer2miRNA.Scer\UAS.5 in PAM neurons under the control of Scer\GAL4Ddc.HL9 results in a significant decline in climbing ability after 49 days.

      (Bou Dib et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Phenotype Manifest In
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The expression of Fer2miRNA.UAS.5 under the control of Scer\GAL4Fer2.2359 enhances the progressive decrease in the number of adult PAM neurons but does not affect the number of adult PAL cluster neurons observed in foxoΔ94 homozygotes.

      (Tas et al., 2018)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Fer2RNAi.UAS.5
      Fer2miRNA.Scer\UAS.5
      Fer2miRNA.UAS.5
      Name Synonyms
      Secondary FlyBase IDs
        References (3)