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General Information
Symbol
Dmel\Fer2MB09480
Species
D. melanogaster
Name
FlyBase ID
FBal0219528
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Fer22
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    Mi{ET1}Fer2MB09480
    Product class / Tool use(s)
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Fer2MB09480 homozygous adults show significant decreases in lifespan and in locomotor capacity (in a startle-induced climbing assay) and a significant and progressive decrease in the number of autophagosomes (i.e. Atg-8-positive puncta) in the PAM neuron region in the adult brain, as compared to heterozygous and wild-type controls.

    The number of PAM dopaminergic neurons is slightly reduced in Fer2MB09480 mutant flies on eclosion, and neuron loss continues progressively at least up to 28 days.

    The majority of visible mitochondria in the cell bodies of remaining PAM neurons in Fer2MB09480 mutant flies are in enlarged aggregations and do not form tubular networks as in th control flies.

    Reactive oxygen levels (ROS) are significantly elevated throughout the brain in 5-day old Fer2MB09480 mutant flies compared with age-matched controls. ROS levels are elevated in PAM dopaminergic neurons. Feeding flies a non-lethal dose of hydrogen peroxide significantly decreases the number of PAM dopaminergic neurons in Fer2MB09480 homozygotes, but not in heterozygotes. Other dopaminergic neuron clusters are unaffected in both homozygotes and heterozygotes.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Suppressed by
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The expression of foxoUAS.cFa under the control of Scer\GAL4GMR58E02 partially suppresses the progressive decrease in the number of adult PAM neurons and the decreased adult locomotion capacity observed in Fer2MB09480 homozygotes, while further reducing their decreased lifespan.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Partially rescued by
    Comments

    Expression of Fer2Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Fer2.2359 fully rescues the climbing defects seen in Fer2MB09480 mutant flies. The PAM dopaminergic loss is also rescued.

    Expression of Fer2Scer\UAS.T:Zzzz\FLAG in PAM neurons under the control of either Scer\GAL4GMR58E02 partially rescues the PAM neuron loss in Fer2MB09480 mutant flies. The climbing defects are also partially rescued.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (3)