FlyBase Allele Report: Dmel\DENR[KO]

General Information

Symbol

Dmel\DENR^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0301001

Feature type

allele

Associated gene

Dmel\DENR

Associated Insertion(s)

TI{TI}DENR^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Schleich et al., 2014)

Mutagen

gene targeting by homologous recombination

(Schleich et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}DENR^KO

Cytology

Description

Part of the DENR gene has been deleted and replaced with a loxP cassette that contains a w^+mW.hs marker.

(Schleich et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:16,886,318..16,886,437 [-]

Comment:

Inferred boundaries of DENR deletion created by homology-mediated recombination. The region was replaced with a mini-white cassette.

(Schleich et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Schleich et al., 2014)

decreased cell number

(Schleich et al., 2014)

increased mortality during development | nutrition conditional

(Vasudevan et al., 2020)

lethal - all die before end of P-stage

(Vasudevan et al., 2020)

lethal - all die during pharate adult stage

(Schleich et al., 2014)

majority die during P-stage

(Vasudevan et al., 2020)

some die during larval stage

(Vasudevan et al., 2020)

visible | P-stage

(Vasudevan et al., 2020)

Phenotype Manifest In

epidermis

(Schleich et al., 2014)

genitalia

(Schleich et al., 2014)

histoblast nest

(Schleich et al., 2014)

leg

(Schleich et al., 2014)

pupa

(Vasudevan et al., 2020)

Detailed Description

Statement

Reference

DENR^KO flies die as pharate adults with a larval-like epidermis due to impaired proliferation of histoblast cells. They have crooked legs and incorrectly rotated genitals. Mutant larvae grow slowly.

(Schleich et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

DENR^KO has lethal - all die during pharate adult stage phenotype, enhanceable by eIF2D^unspecified

(Schleich et al., 2014)

Suppressed by

Statement

Reference

DENR^KO has abnormal developmental rate phenotype, suppressible | partially by EcR^UAS.cUa/Scer\GAL4^esg.PU

(Schleich et al., 2014)

DENR^KO has abnormal developmental rate phenotype, suppressible | partially by InR^UAS.cUa/Scer\GAL4^esg.PU

(Schleich et al., 2014)

Other

Statement

Reference

DENR^KO, eIF2D^CR1 has some die during larval stage phenotype

(Vasudevan et al., 2020)

DENR^KO, eIF2D^CR1 has lethal - all die before end of P-stage phenotype

(Vasudevan et al., 2020)

DENR^KO, eIF2D^CR1 has majority die during P-stage phenotype

(Vasudevan et al., 2020)

DENR^KO, eIF2D^CR1 has visible | P-stage phenotype

(Vasudevan et al., 2020)

DENR^KO, eIF2D^CR1 has increased mortality during development | nutrition conditional phenotype

(Vasudevan et al., 2020)

Phenotype Manifest In

Suppressed by

Statement

Reference

DENR^KO has histoblast nest phenotype, suppressible | partially by EcR^UAS.cUa/Scer\GAL4^esg.PU

(Schleich et al., 2014)

DENR^KO has histoblast nest phenotype, suppressible | partially by InR^UAS.cUa/Scer\GAL4^esg.PU

(Schleich et al., 2014)

Other

Statement

Reference

DENR^KO, eIF2D^CR1 has pupa phenotype

(Vasudevan et al., 2020)

DENR^KO, eIF2D^CR1 has anterior pupal spiracle | absent phenotype

(Vasudevan et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

DENR^KO is rescued by Scer\GAL4^Tub.PU/DENR^UAS.cSa

(Schleich et al., 2014)

DENR^KO is rescued by DENR^UAS.cSa/Scer\GAL4^esg.PU

(Schleich et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76341

TI{TI}DENR^KO/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

DENR^KO

(Vasudevan et al., 2020, Schleich et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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