FlyBase Allele Report: Dmel\mir-965[KO1]

General Information

Symbol

Dmel\mir-965^KO1

Species

D. melanogaster

Name

FlyBase ID

FBal0301939

Feature type

allele

Associated gene

Dmel\mir-965

Associated Insertion(s)

TI{TI}mir-965^KO1

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Verma and Cohen, 2015, Chen et al., 2014)

Mutagen

ends-out gene targeting

(Chen et al., 2014)

FLPase

(Chen et al., 2014)

I-SceI

(Chen et al., 2014)

cre recombinase

(Chen et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-965^KO1

Cytology

Description

The mir-965 hairpin has been deleted. A single loxP site is present at the site of the deletion. The expression of the kis host gene appears unaffected (assayed by quantitative PCR).

(Chen et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:243,050..243,199 [-]

Comment:

Region containing the mir-965 hairpin is deleted via homologous recombination and replaced with a mini-white cassette. The deleted region was inferred from primer sequences.

(Verma and Cohen, 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile

(Chen et al., 2014)

long lived | female | recessive

(Chen et al., 2014)

long lived | male | recessive

(Chen et al., 2014)

male fertile

(Chen et al., 2014)

short lived | female (with Df(2L)ED19)

(Chen et al., 2014)

short lived | male (with Df(2L)ED19)

(Chen et al., 2014)

viable

(Verma and Cohen, 2015, Chen et al., 2014)

viable (with Df(2L)ED19)

(Verma and Cohen, 2015)

viable (with mir-965^KO2)

(Verma and Cohen, 2015)

visible

(Verma and Cohen, 2015)

visible (with mir-965^KO2)

(Verma and Cohen, 2015)

Phenotype Manifest In

abdomen

(Verma and Cohen, 2015)

abdomen (with mir-965^KO2)

(Verma and Cohen, 2015)

epidermal cell | ectopic | larval stage (with mir-965^KO2)

(Verma and Cohen, 2015)

histoblast (with mir-965^KO2)

(Verma and Cohen, 2015)

histoblast nest (with mir-965^KO2)

(Verma and Cohen, 2015)

Detailed Description

Statement

Reference

mir-965^KO1 mutant flies appear to be morphologically normal with the exception of defects in abdominal segmentation. The predominant defect is a dorsal gap in one or more abdominal segments, in some cases leading to segment fusion. Some of these flies also exhibit ectopic bristles associated with a polarity defect.

mir-965^KO1/mir-965^KO2 mutant flies appear to be morphologically normal with the exception of defects in abdominal segmentation. The predominant defect is a dorsal gap in one or more abdominal segments, in some cases leading to segment fusion. Some of these flies also exhibit ectopic bristles associated with a polarity defect.

mir-965^KO1/mir-965^KO2 mutant histoblasts undergo asynchronous division, resulting in the presence of cells of different sizes. In contrast to controls, a subset of the histoblasts undergo cell death. Subsequently the rate of histoblast nest expansion is slower in the mir-965^KO1/mir-965^KO2 mutants than in controls. In contrast to wild type, larval epidermal cells are still present in the nests.

(Verma and Cohen, 2015)

Homozygous males and females show a significant increase in median lifespan compared to controls.

mir-965^KO/Df(2L)ED19 males and females show a significant decrease in median lifespan compared to controls.

(Chen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-965^KO1/mir-965^KO2 has visible phenotype, suppressible | partially by wg^Sp-1/wg[+]/stg⁴/stg[+]

(Verma and Cohen, 2015)

mir-965^KO1/mir-965^KO2 has visible phenotype, suppressible | partially by stg[+]/stg^EY12388

(Verma and Cohen, 2015)

mir-965^KO1/mir-965^KO2 has visible phenotype, suppressible | partially by stg⁴/stg[+]

(Verma and Cohen, 2015)

mir-965^KO1/mir-965^KO2 has visible phenotype, suppressible | partially by wg^Sp-1/wg[+]

(Verma and Cohen, 2015)

NOT suppressed by

Statement

Reference

mir-965^KO1/mir-965^KO2 has visible phenotype, non-suppressible | partially by wg^Sp-1/wg[+]

(Verma and Cohen, 2015)

Other

Statement

Reference

kis[+]/kis¹, mir-965^KO1 has viable phenotype

(Verma and Cohen, 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

mir-965^KO1/mir-965^KO2 has abdomen phenotype, suppressible | partially by stg[+]/stg^EY12388

(Verma and Cohen, 2015)

mir-965^KO1/mir-965^KO2 has histoblast phenotype, suppressible | partially by stg[+]/stg^EY12388

(Verma and Cohen, 2015)

mir-965^KO1/mir-965^KO2 has abdomen phenotype, suppressible | partially by stg⁴/stg[+]

(Verma and Cohen, 2015)

mir-965^KO1/mir-965^KO2 has abdomen phenotype, suppressible | partially by wg^Sp-1/wg[+]

(Verma and Cohen, 2015)

mir-965^KO1/mir-965^KO2 has abdomen phenotype, suppressible | partially by wg^Sp-1/wg[+]/stg⁴/stg[+]

(Verma and Cohen, 2015)

NOT suppressed by

Statement

Reference

mir-965^KO1/mir-965^KO2 has abdomen phenotype, non-suppressible | partially by wg^Sp-1/wg[+]

(Verma and Cohen, 2015)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of stg^EY12388 partially suppresses the segment gap phenotype seen in the abdomens of mir-965^KO1/mir-965^KO2 mutant flies. The asynchronous histoblast division and reduction in histoblast migration rate are also suppressed.

One copy of stg⁴ partially suppresses the segment gap phenotype seen in the abdomens of mir-965^KO1/mir-965^KO2 mutant flies. The asynchronous histoblast division and reduction in histoblast migration rate are also suppressed.

One copy of wg^Sp-1 partially suppresses the segment gap phenotype seen in the abdomens of mir-965^KO1/mir-965^KO2 mutant flies.

One copy of wg^l-12 does not significantly suppress the segment gap phenotype seen in the abdomens of mir-965^KO1/mir-965^KO2 mutant flies.

One copy each of stg⁴ and wg^Sp-1 partially suppresses the segment gap phenotype seen in the abdomens of mir-965^KO1/mir-965^KO2 mutant flies.

(Verma and Cohen, 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58946

w^*; TI{TI}mir-965^KO1/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

miR-965^K01

(Klaustermeier et al., 2025)

mir-965^KO1

(Verma and Cohen, 2015)

mir-965^KO

mir-965^w-KO1

(Verma and Cohen, 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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