FlyBase Allele Report: Dmel\mir-124[KO]

General Information

Symbol

Dmel\mir-124^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0316238

Feature type

allele

Associated gene

Dmel\mir-124

Associated Insertion(s)

TI{TI}mir-124^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Wang et al., 2014)

Mutagen

ends-out gene targeting

(Wang et al., 2014)

FLPase

(Wang et al., 2014)

I-SceI

(Wang et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-124^KO

Cytology

Description

A 1.6kb genomic fragment which includes the 99bp pre-mir-124 hairpin has been replaced by a loxP cassette containing a w^+mW.hs marker.

(Wang et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:17,565,650..17,567,291 [+]

Comment:

1.6 kb deletion of the mir-124 locus created by ends-out gene targeting. Deletion boundaries inferred from primer sequences.

(Wang et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight

(Wang et al., 2014)

abnormal locomotor behavior | adult stage

(Wang et al., 2014)

abnormal neuroanatomy | third instar larval stage

(Wang et al., 2014)

female semi-sterile

(Wang et al., 2014)

Phenotype Manifest In

dendrite

(Wang et al., 2014)

embryonic/larval neuromuscular junction | third instar larval stage

(Wang et al., 2014)

larval dorsal multidendritic neuron ddaE | third instar larval stage

(Wang et al., 2014)

type Ib terminal bouton

(Wang et al., 2014)

type Is terminal bouton

(Wang et al., 2014)

Detailed Description

Statement

Reference

Homozygous adults show significantly reduced ability in a climbing assay compared to controls and only 5% of adults can fly.

Homozygous third instar larvae show a significant reduction in branch length and the number of both type Ib and Is boutons at neuromuscular junction (NMJ) 6/7 compared to controls (significant differences to controls are not seen at NMJ 4).

The ddaE dendritic arborization neurons show a significant reduction in the number of dendritic branches in homozygous third instar larvae.

(Wang et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

mir-124^KO is partially rescued by Scer\GAL4^elav.PU/mir-124^UAS.cWa

(Wang et al., 2014)

Comments

The reduced climbing and flight abilities of homozygous mir-124^KO adults are partly rescued by expression of mir-124^Scer\UAS.cWa under the control of Scer\GAL4^elav.PU. The reduced fertility of homozygous mir-124^KO females is also rescued.

(Wang et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mir-124^KO

(Wang et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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