FlyBase Allele Report: Scer\GAL4[VP16.Lgr3]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Scer\GAL4^VP16.Lgr3

General Information

Symbol

Scer\GAL4^VP16.Lgr3

Species

S. cerevisiae

Name

FlyBase ID

FBal0317079

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{Lgr3-GAL4::VP16}

PBac{Lgr3-GAL4::VP16}

Also Known As

lgr3-GAL4, Lgr3-GAL4::VP16

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Colombani et al., 2015)

Progenitor genotype

Carried in construct

P{Lgr3-GAL4::VP16}

PBac{Lgr3-GAL4::VP16}

Cytology

Description

Genomic fragment (derived from BAC clone CH322-155A11) containing the entire Lgr3 locus, in which the coding portion of the first Lgr3 exon has been replaced with sequence which encodes a GAL4::VP16 driver followed by the Hsp70 3'UTR.

(Colombani et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage | male

larval brain | restricted

(Meissner et al., 2016)

second instar larval stage | female

larval brain | intense | restricted

(Meissner et al., 2016)

second instar larval stage

larval ventral nerve cord | restricted

(Meissner et al., 2016)

early third instar larval stage | male

larval brain | restricted

(Meissner et al., 2016)

early third instar larval stage | female

larval brain | intense | restricted

(Meissner et al., 2016)

early third instar larval stage

larval ventral nerve cord | restricted

(Meissner et al., 2016)

late third instar larval stage

larval neuron | subset of larval central nervous system

(Colombani et al., 2015)

larval ventral nerve cord | restricted

(Meissner et al., 2016)

late third instar larval stage | male

larval brain | restricted

(Meissner et al., 2016)

late third instar larval stage | female

larval brain | intense | restricted

(Meissner et al., 2016)

pupal stage P6 | male

adult median bundle

Comment: ~21hrs APF

(Meissner et al., 2016)

pupal stage P6 | female

adult median bundle | intense

Comment: ~21hrs APF

(Meissner et al., 2016)

adult abdominal neuromere | restricted

Comment: ~21hrs APF

(Meissner et al., 2016)

pupal stage P7 | male

adult median bundle

Comment: ~43hrs APF

(Meissner et al., 2016)

pupal stage P7 | female

adult median bundle | intense

Comment: ~43hrs APF

(Meissner et al., 2016)

adult abdominal neuromere | restricted

Comment: ~43hrs APF

(Meissner et al., 2016)

pharate adult stage P9 | male

adult median bundle

Comment: ~71hrs APF

(Meissner et al., 2016)

pharate adult stage P9 | female

adult median bundle | intense

Comment: ~71hrs APF

(Meissner et al., 2016)

pharate adult stage P9

adult abdominal neuromere | restricted

Comment: ~71hrs APF

(Meissner et al., 2016)

pharate adult stage P14 | male

adult median bundle

Comment: ~90hrs APF

(Meissner et al., 2016)

pharate adult stage P14 | female

adult median bundle | intense

Comment: ~90hrs APF

(Meissner et al., 2016)

pharate adult stage P14

adult abdominal neuromere | restricted

Comment: ~90hrs APF

(Meissner et al., 2016)

adult stage | male

adult median bundle

(Meissner et al., 2016)

adult abdominal neuromere | restricted

(Meissner et al., 2016)

adult stage | female

adult median bundle | intense

(Meissner et al., 2016)

adult abdominal neuromere | restricted

(Meissner et al., 2016)

Additional Information

Statement

Reference

ScerGAL4^VP16.Lgr3 drives sexually dimorphic expression in the median bundle, where it is expressed more strongly and in more neurons in the female than in the male in pupae and adults, and in the abdominal neuromere. Expression overlaps completely with an Lgr3 antisense probe., except in the adult abdominal neuromere where the probe is expressed in a few more cells. Expression also overlaps with EcollexA^fru.P1 and EcollexA^GMR19B09 in females, but less so in males.

(Meissner et al., 2016)

Marker for

Reflects expression of

(Meissner et al., 2016, Colombani et al., 2015)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Lgr3^GL01056/Scer\GAL4^VP16.Lgr3 is a suppressor of abnormal developmental rate phenotype of Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa

(Colombani et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Lgr3^GL01056/Scer\GAL4^VP16.Lgr3 is a suppressor of wing disc phenotype of Ecol\lexA^rn-89-lexA, Ilp8^lexAop.cCa

(Colombani et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

66683

w^*; P{Lgr3-GAL4::VP16}attP40/CyO

66684

w^*; sp^*; PBac{Lgr3-GAL4::VP16}VK00033

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{Lgr3.T:Hsim\VP16}

Scer\GAL4^VP16.Lgr3

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections