FlyBase Allele Report: Dmel\WRNexo[Δ]

General Information

Symbol

Dmel\WRNexo^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0318470

Feature type

allele

Associated gene

Dmel\WRNexo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bolterstein et al., 2014)

Mutagen

P-element activity

(Bolterstein et al., 2014)

Progenitor genotype

P{EP}G16048

(Bolterstein et al., 2014)

Cytology

Description

2.5kb deletion that removes most of the WRNexo coding sequence (deletion extends from 426bp upstream of the 5'UTR to 17bp upstream of the 3'UTR). Generated by imprecise excision of P{EP}G16048. Expression of the upstream Nup43 gene is not affected.

(Bolterstein et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:18,364,260..18,366,588 [-]

Comment:

Inferred boundaries of a 2.5 kb deletion resulting from the imprecise excision of P{EP}G16048, which is reported to delete sequences from 426 bp upstream of the 5'-UTR to 17 bp upstream of the 3'-UTR. The coordinates used for the ends of the UTRs were not defined.

(Bolterstein et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Werner syndrome

CEA

(Sember et al., 2024, Epiney et al., 2021, Cassidy et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | embryonic stage

(Bolterstein et al., 2014)

fertile

(Bolterstein et al., 2014)

partially lethal

(Bolterstein et al., 2014)

short lived | chemical conditional

(Bolterstein et al., 2014)

short lived | chemical conditional (with Df(3R)Exel6178)

(Bolterstein et al., 2014)

some die during embryonic stage

(Bolterstein et al., 2014)

viable

(Bolterstein et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

WRNexo^Δ/WRNexo^Δ flies have normal survival rates in response to camptothecin or topotecan treatment and are not sensitive to bleomycin.

WRNexo^Δ/WRNexo^Δ mutants have significantly reduced hatching frequency and embryos have more nuclear division defects (increased number of anaphase bridges and gaps between nuclei) and significantly increased double-strand breaks compared to wild type.

Larval exposure to hydroxyurea decreases (dose-dependently) WRNexo^Δ/WRNexo^Δ or WRNexo^Δ/Df(3R)Exel6178 adult survival rates.

WRNexo^Δ/WRNexo^D229V mutants do not have reduced hatching frequency or hydroxyurea sensitivity (except hatching frequency is significantly reduced when embryos are laid by WRNexo^Δ females mated with WRNexo^D229V/WRNexo^Δ males).

(Bolterstein et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

WRNexo^Δ has short lived | chemical conditional phenotype, enhanceable by spn-A³/spn-A³

(Bolterstein et al., 2014)

NOT Enhanced by

Statement

Reference

WRNexo^Δ has short lived | chemical conditional phenotype, non-enhanceable by Blm^N1/Blm^N1

(Bolterstein et al., 2014)

Other

Statement

Reference

WRNexo^Δ, mus81^Nhe has short lived phenotype

(Bolterstein et al., 2014)

WRNexo^Δ, mus81^Nhe has partially lethal phenotype

(Bolterstein et al., 2014)

WRNexo^Δ, mus312^D1 has lethal - all die during pharate adult stage phenotype

(Bolterstein et al., 2014)

Gen^Z4325, WRNexo^Δ has lethal - all die during P-stage phenotype

(Bolterstein et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

spn-A³/spn-A³ enhances hydroxyurea sensitivity (decreases adult survival) in WRNexo^Δ/WRNexo^Δ mutants. Blm^N1/Blm^N1 WRNexo^Δ/WRNexo^Δ double mutants have similar hydroxyurea sensitivity to either single mutant.

Synthetic lethality is observed in double mutants: WRNexo^Δ/WRNexo^Δ mus81^Nhe/mus81^Nhe survive to adulthood but homozygotes eclose at lower frequencies than expected and show poor survival; WRNexo^Δ/WRNexo^Δ mus312^D1/mus312^D1 survive to pharate adult stage; WRNexo^Δ/WRNexo^Δ Gen^Z4325/Gen^Z4325 survive to pupal stage. WRNexo^D229V/WRNexo^D229V mus81^Nhe/mus81^Nhe double mutants survive to adulthood and are healthy.

(Bolterstein et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

WRNexo^Δ

(Sember et al., 2024, Epiney et al., 2021, Cassidy et al., 2019, Bolterstein et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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