UAS regulatory sequences drive expression of Gyc76C which lacks the C-terminal domain (amino acids 1105-1521 are deleted). The endogenous signal peptide sequence has been replaced by a Igk-leader sequence followed by 5 copies of the Tag:MYC tag.
Scer\GAL4elav.PU/Gyc76CΔCterm.UAS.Tag:MYC fails to rescue Gyc76CKG03723ex173
Expression of Gyc76CΔCterm.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4elav.PU fails to rescue the ISNb and SNa motor neuron axon pathway phenotypes of Gyc76CKG03723ex173/Gyc76CKG03723ex173 embryos.