UASt regulatory sequences drive expression of Gabat (derived from the RH42429 cDNA clone), that is tagged at the C-terminal end with Tag:V5-Tag:polyHis.
GabatUAS.Tag:V5,Tag:polyHis, Gabatf01602, Scer\GAL4Gabat-PL00338, qvrEY04063 has abnormal sleep phenotype
GabatUAS.Tag:V5,Tag:polyHis, Gabatf01602, Scer\GAL4repo.PU, qvrEY04063 has abnormal sleep phenotype
Expression of GabatScer\UAS.T:SV5\V5,T:Zzzz\His6 driven by Scer\GAL4Gabat-PL00338 or Scer\GAL4repo.PU (but not Scer\GAL4Gad1.3.098) in a qvrEY04063/qvrEY04063;Gabatf01602/Gabatf01602 double mutant background diminishes sleep to around 35% (sleep is also more fragmented and sleep latency increases, whereas sleep bout number is unchanged) of qvrEY04063/qvrEY04063;Gabatf01602/Gabatf01602 (partial rescue).
Expression of GabatScer\UAS.T:SV5\V5,T:Zzzz\His6 under the control of Scer\GAL4elav.PU or Scer\GAL4repo.PU rescues the reduced survival of Gabatf01602/Gabatf01602 mutants raised on a sucrose only diet.