FlyBase Allele Report: Dmel\qvr[EY04063]

qvr^EY04063/qvr^EY04063 males sleep significantly less than wild type during both day and night and have shorter sleep bout durations and increased bout numbers, as well as increases in sleep latency. Mutants display weak circadian activity rhythms under constant dark conditions.

Ethanolamine O-sulfate treatment (10mM in adult males, to inhibit GABAT) partially rescues nighttime sleep in qvr^EY04063/qvr^EY04063 mutants.

(Chen et al., 2015)

qvr^EY04063 mutants display normal wing beat frequency and DLM firing rate. However, the DLM spiking pattern in these flies lacks regularity (as measured by the CV of inter-spike intervals).

(Iyengar and Wu, 2014)

Homozygous and qvr^EY04063/qvr^f01257 adults show a reduced amount of daily sleep compared to controls.

(Wu et al., 2014)

The spontaneous miniature excitatory junctional potential (mEJP) frequency is significantly increased in qvr^EY04063 mutant larvae compared with wild-type controls.

The magnitude and kinetics of the Sh-dependent I[[A]] current in larval muscles is altered in qvr^EY04063 mutants. The qvr^EY04063 mutation significantly delayed the time to peak of I[[A]] at 10 mV and also decreased the I[[A]] current magnitude at every voltage greater than 20 mV.

(Wu et al., 2010)

Male and female homozygous flies show a severe reduction in both daytime and nighttime sleep compared to controls; the reduction is more than 85% for males and more than 80% for females. Approximately 9% of the mutant flies do not sleep at all. Despite the reduction in daily sleep, waking activity is not significantly elevated. The reduction in sleep is largely due to a sharp reduction in the duration of sleep bouts, but is also attributable in part to a significant reduction in the number of daily sleep bouts. Heterozygotes behave similarly to controls in their sleep behaviour.

qvr^Δ40/qvr^EY04063 flies show a severely reduced amount of daily sleep compared to control flies.

qvr^EY04063/qvr^f01257 flies have a 30% reduction in daily sleep relative to control qvr^EY04063/+ flies.

Mechanical stimulation results in moderate reduction in the amount of sleep lost in qvr^EY04063/qvr^f01257 flies compared to controls. These flies show little or no rebound after the sleep deprivation.

Homozygous flies show weak locomotor activity circadian rhythms under constant darkness conditions.

Almost all qvr^EY04063/qvr^f01257 flies are rhythmic for circadian locomotor activity.

Homozygous flies show normal phototactic responses and perform as well as controls in a taste discrimination assay. Homozygotes do not show a bang sensitive phenotype, but they are somewhat uncoordinated.

Homozygotes have a shortened life span compared to controls.

Homozygous and qvr^EY04063/qvr¹ flies show ether-induced leg shaking.

qvr^EY04063/qvr¹ flies show a significant decrease in sleep relative to wild-type controls.

(Koh et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

qvr^Δ40/qvr^EY04063 has decreased sleep | adult stage phenotype, suppressible by Gabat^PL00338

(Zhou et al., 2023)

qvr^Δ40/qvr^EY04063 has increased cell number | adult stage phenotype, suppressible by Gabat^PL00338

(Zhou et al., 2023)

qvr^EY04063 has abnormal sleep phenotype, suppressible by Gabat^f01602/Gabat^f01602

(Chen et al., 2015)

qvr^EY04063 has abnormal sleep phenotype, suppressible by Gabat^PL00338/Gabat^PL00338

(Chen et al., 2015)

qvr^EY04063 has abnormal locomotor rhythm phenotype, suppressible by Gabat^f01602/Gabat^f01602

(Chen et al., 2015)

qvr^EY04063 has abnormal sleep phenotype, suppressible by Scer\GAL4^qvr.PW/Mmus\Lynx1^UAS.cWa

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, suppressible by nAChRβ3^JF01947/Scer\GAL4^qvr.PW

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, suppressible by Scer\GAL4^qvr.PW/nAChRα3^JF02750

(Wu et al., 2014)

NOT suppressed by

Statement

Reference

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRα1^JF03103

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRα2^JF02643

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRα4^JF03419

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRα5^JF01963

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRα6^JF01853

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRα7^JF02570

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRβ1^JF02158

(Wu et al., 2014)

qvr^EY04063 has abnormal sleep phenotype, non-suppressible by Scer\GAL4^qvr.PW/nAChRβ2^JF02873

(Wu et al., 2014)

NOT Enhancer of

Statement

Reference

qvr[+]/qvr^EY04063 is a non-enhancer of abnormal sleep | dominant phenotype of nAChRα4^rye

(Shi et al., 2014)

Other

Statement

Reference

Gabat^{UAS.Tag:V5,Tag:polyHis}, Gabat^f01602, Scer\GAL4^{Gabat-PL00338}, qvr^EY04063 has abnormal sleep phenotype

(Chen et al., 2015)

Gabat^{UAS.Tag:V5,Tag:polyHis}, Gabat^f01602, Scer\GAL4^repo.PU, qvr^EY04063 has abnormal sleep phenotype

(Chen et al., 2015)

Gabat^{UAS.Tag:V5,Tag:polyHis}, Gabat^f01602, Scer\GAL4^Gad1.3.098, qvr^EY04063 has abnormal sleep phenotype

(Chen et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

qvr^Δ40/qvr^EY04063 has intestinal stem cell | increased number phenotype, suppressible by Gabat^PL00338

(Zhou et al., 2023)

qvr^Δ40/qvr^EY04063 has adult gut phenotype, suppressible | partially by Gabat^PL00338

(Zhou et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Gabat^f01602/Gabat^f01602 partially suppresses sleep phenotypes seen in qvr^EY04063/qvr^EY04063 flies; in addition, double mutants show an increase in the fraction of long sleep episodes and reductions in nighttime sleep bout numbers (similar to Gabat^f01602/Gabat^f01602 single mutants); qvr^EY04063/qvr^EY04063 circadian rhythmicity is increased with the introduction of Gabat^f01602/Gabat^f01602. Stronger suppression of the short sleep phenotype in qvr^EY04063/qvr^EY04063 flies is seen with the introduction of Gabat^PL00338/Gabat^PL00338.

Expression of Gabat^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} driven by Scer\GAL4^{Gabat-PL00338} or Scer\GAL4^repo.PU (but not Scer\GAL4^Gad1.3.098) in a qvr^EY04063/qvr^EY04063;Gabat^f01602/Gabat^f01602 double mutant background diminishes sleep to around 35% (sleep is also more fragmented and sleep latency increases, whereas sleep bout number is unchanged) of qvr^EY04063/qvr^EY04063;Gabat^f01602/Gabat^f01602 (partial rescue).

(Chen et al., 2015)

qvr^EY04063/+ ; wake^GS17103/+ and qvr^EY04063/+ ; Rdl¹/+ double heterozygotes show no significant change in sleep latency compared to controls.

(Liu et al., 2014)

nAChRα4^rye/+; qvr^EY04063/+ flies show sleep duration comparable to that of nAChRα4^rye/+ alone.

(Shi et al., 2014)

Expression of either nAChRα3^JF02750 or nAChRβ3^JF01947 under the control of Scer\GAL4^qvr.PW partially rescues the reduction in total amount of daily sleep which is seen in qvr^EY04063 flies.

Expression of any one of nAChRα1^JF03103, nAChRα2^JF02643, nAChRα4^JF03419, nAChRα5^JF01963, nAChRα6^JF01853, nAChRα7^JF02570, nAChRβ1^JF02158 or nAChRβ2^JF02873 under the control of Scer\GAL4^qvr.PW does not rescue the reduction in total amount of daily sleep which is seen in qvr^EY04063 flies.

(Wu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Lynx1^Scer\UAS.cWa under the control of Scer\GAL4^qvr.PW partially rescues the reduced daily sleep seen in qvr^EY04063 flies, without altering waking activity.

(Wu et al., 2014)

Complementation and Rescue Data

Fails to complement

qvr^Δ40

(Koh et al., 2008)

qvr¹

(Koh et al., 2008)

Rescued by

qvr^Δ40/qvr^EY04063 is rescued by qvr^UAS.cWa/Scer\GAL4^elav.PLu

(Zhou et al., 2023)

qvr^Δ40/qvr^EY04063 is rescued by qvr^UAS.cWa/Scer\GAL4^nSyb.PS

(Zhou et al., 2023)

qvr^Δ40/qvr^EY04063 is rescued by Scer\GAL4^Tub.PU/qvr^UAS.cWa

(Zhou et al., 2023)

qvr^EY04063 is rescued by Scer\GAL4^qvr.PW/qvr^UAS.cWa

(Wu et al., 2014)

qvr^EY04063 is rescued by Scer\GAL4^qvr.PW/qvr^UAS.cWa

(Wu et al., 2010)

qvr^EY04063 is rescued by qvr^+tKa

(Koh et al., 2008)

Partially rescued by

qvr^EY04063 is partially rescued by Scer\GAL4^{elav.Switch.PO}/qvr^UAS.cWa

(Chen et al., 2015)

qvr^EY04063 is partially rescued by Scer\GAL4^Gad1.3.098/qvr^UAS.cWa

(Chen et al., 2015)

qvr^EY04063 is partially rescued by Scer\GAL4^VGAT.PF/qvr^UAS.cWa

(Chen et al., 2015)

qvr^EY04063 is partially rescued by Scer\GAL4^repo.PU/qvr^UAS.cWa

(Chen et al., 2015)

qvr^EY04063 is partially rescued by Scer\GAL4^elav-C155/qvr^UAS.cWa

(Wu et al., 2010)

qvr^EY04063 is partially rescued by qvr^UAS.cWa/Scer\GAL4^ChAT.7.4

(Wu et al., 2010)

qvr^EY04063 is partially rescued by Scer\GAL4^how-24B/qvr^UAS.cWa

(Wu et al., 2010)

qvr^EY04063 is partially rescued by qvr^UAS.cWa/Scer\GAL4^VGlut1.PS

(Wu et al., 2010)

Not rescued by

qvr^EY04063 is not rescued by Scer\GAL4^{Gabat-PL00338}/qvr^UAS.cWa

(Chen et al., 2015)

qvr^EY04063 is not rescued by Scer\GAL4^ple.PF/qvr^UAS.cWa

(Wu et al., 2010)

Comments

Restoring qvr^Scer\UAS.cWa specifically in adults (driven by Scer\GAL4^{elav.Switch.PO} with RU486 feeding) partially rescues qvr^EY04063/qvr^EY04063 sleep phenotypes.

Expressing qvr^Scer\UAS.cWa driven by Scer\GAL4^Gad1.3.098 or Scer\GAL4^VGAT.PF significantly restores sleep to 80-90% (respectively) of wild type levels in qvr^EY04063/qvr^EY04063 flies; rescued sleep is due to both lengthened sleep bout duration and increased bout number and sleep latency is returned to wild type levels.

Expressing qvr^Scer\UAS.cWa driven by Scer\GAL4^repo.PU or Scer\GAL4^{Gabat-PL00338} does not rescue sleep phenotypes (no significant changes in nighttime sleep, sleep bout duration or number, or sleep latency) in qvr^EY04063/qvr^EY04063 flies; when driven by Scer\GAL4^repo.PU, there is a slight (significant) increase in day time sleep.

(Chen et al., 2015)

Expression of qvr^Scer\UAS.cWa under the control of Scer\GAL4^qvr.PW completely rescues the reduced daily sleep seen in qvr^EY04063 flies.

(Wu et al., 2014)

The amount of daily sleep returns to wild-type levels in qvr^EY04063 mutant flies carrying both the Scer\GAL4^qvr.PW driver and the qvr^Scer\UAS.cWa transgene.

Pan-neural expression of qvr^Scer\UAS.cWa under the control of the Scer\GAL4^elav-C155 driver fully rescues the qvr^EY04063 mutant sleep phenotype.

Expressing qvr^Scer\UAS.cWa in cholinergic neurons using Scer\GAL4^Cha.7.4 results in strong rescue of the short-sleeping phenotype (but not the leg-shaking) of qvr^EY04063 mutants.

Scer\GAL4^VGlut.PS- or Scer\GAL4^ple.PF-driven expression of qvr^Scer\UAS.cWa results in weak or no rescue of the qvr^EY04063 mutant sleep phenotype.

The leg-shaking phenotype of qvr^EY04063 mutants is rescued by Scer\GAL4^elav-C155- or Scer\GAL4^qvr.PW-driven expression of qvr^Scer\UAS.cWa.

Scer\GAL4^VGlut.PS driven expression of qvr^Scer\UAS.cWa rescues the qvr^EY04063 mutant leg-shaking phenotype.

Presynaptic expression of qvr^Scer\UAS.cWa driven by Scer\GAL4^elav-C155 rescues the spontaneous miniature excitatory junctional potential (mEJP) frequency phenotype of qvr^EY04063 mutants.

Expression of qvr^Scer\UAS.cWa driven by either Scer\GAL4^how-24B or Scer\GAL4^Cha.7.4 fails to rescue the spontaneous miniature excitatory junctional potential (mEJP) frequency phenotype of qvr^EY04063 mutants.

Expression of qvr^Scer\UAS.cWa driven by Scer\GAL4^how-24B rescues the Sh-dependent I[[A]] current kinetics and amplitude phenotypes of qvr^EY04063 mutants.

Expression of qvr^Scer\UAS.cWa driven by Scer\GAL4^elav-C155 fails to rescue the Sh-dependent I[[A]] current kinetics and amplitude phenotypes of qvr^EY04063 mutants.

(Wu et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

16588

y¹ w^67c23; P{EPgy2}qvr^EY04063/CyO

Notes on Origin

Discoverer

Comments

Precise excision of the insertion in qvr^EY04063 restores the daily sleep amount to wild-type levels.

(Koh et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG33472^EY04063

qvr^EY04063

(Vaughen et al., 2022)

qvr^EY

(Ruan et al., 2017, Iyengar and Wu, 2014)

sleepless^P1

(Axelrod et al., 2023)

sss(EY04063)

(Huang et al., 2025)

sss^P1

(Zhou et al., 2023, Huang et al., 2020, Öztürk-Çolak et al., 2020, Chen et al., 2015, Liu et al., 2014, Shi et al., 2014, Wu et al., 2014, Wu et al., 2010, Koh et al., 2008)

sss^p1

(Cuddapah et al., 2025)

Name Synonyms

Secondary FlyBase IDs

References (16)

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