UAS regulatory sequences drive expression of a polycistronic cDNA encoding the wild-type hemo and CG43210 open reading frames. A GFP tag has been inserted at the C-terminal end of the hemo open reading frame.
hemoUAS.FL.GFP/Scer\GAL4crq.PO rescues Df(3R)hemo-A4
hemoUAS.FL.GFP/Scer\GAL4He.PZ rescues Df(3R)hemo-A4
Expression of hemoScer\UAS.FL.T:Avic\GFP under the control of Scer\GAL4crq.PO or Scer\GAL4He.PZ rescues the vacuolation and increased acidic compartments seen in hemo-A4/hemo-A4 mutant hemocytes.