FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4Gr66a.PU
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General Information
Symbol
Scer\GAL4Gr66a.PU
Species
S. cerevisiae
Name
Gustatory receptor 66a promoter construct of Unknown
FlyBase ID
FBal0320346
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Gr66a-GAL4.U}
Also Known As
Gr66a-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(FlyBase Curators, 2025-)
Progenitor genotype
Carried in construct
P{Gr66a-GAL4.U}
Cytology
Description

Gr66a regulatory sequences regulate expression of a GAL4 driver.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)
Allele components
Component
Use(s)
Regulatory region(s)
Gr66a
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      gustatory receptor neuron B1 of the terminal organ dorsolateral group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron B2 of the terminal organ dorsolateral group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C1 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C2 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C3 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      gustatory receptor neuron C4 of the terminal organ distal group
      (Apostolopoulou et al., 2016)
      larval dorsal pharyngeal gustatory receptor neuron DP1
      (Apostolopoulou et al., 2016)
      larval dorsal pharyngeal gustatory receptor neuron DP2
      (Apostolopoulou et al., 2016)
      larval ventral pharyngeal gustatory receptor neuron VP1/VP2
      (Apostolopoulou et al., 2016)
      larval posterior pharyngeal gustatory receptor neuron PP1
      (Apostolopoulou et al., 2016)
      larval posterior pharyngeal gustatory receptor neuron PP2
      (Apostolopoulou et al., 2016)
      adult stage
      neuron of adult subesophageal zone | restricted
      (Hussain et al., 2016)
      neuron of labellum | restricted
      (Hussain et al., 2016)
      neuron of adult antennal lobe | restricted
      (Hussain et al., 2016)
      neuron of leg | restricted
      (Hussain et al., 2016)
      large labellar taste bristle
      (Hussain et al., 2016)
      labellar taste peg
      (Hussain et al., 2016)
      neuron of proboscis | restricted
      (Hussain et al., 2016)
      Additional Information
      Statement
      Reference

      Expression of UAS- mCD8::GFP via ScerGAL4Gr66a.PU confirmed that it is expressed in 12 GRN pairs. These 12 pairs are suggested to represent the entire set of larval bitter receptor neurons. They are: (i) two pairs B1 and B2 in the dorsolateral group of sensilla of the TO, (ii) four pairs C1-C4 in the distal group of TO sensilla, (iii) two pairs D1 and D2 in the DPS (epiphysis), (iv) two pairs E1 and E2 in the VPS (hypophysis), and (v) two pairs F1 and F2 in the PPS (hypopharyngeal organ).

      (Apostolopoulou et al., 2016)
       
      Marker for
      Reflects expression of
      Gr66a
      (Apostolopoulou et al., 2016, Hussain et al., 2016)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4Gr66a.PU/Ir76bUAS.cZa fails to rescue Ir76b1

      (Hussain et al., 2016)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Gr66aGal4
      (Masuzzo et al., 2022)
      Scer\GAL4Gr66a.PU
      Name Synonyms
      Gustatory receptor 66a promoter construct of Unknown
      Secondary FlyBase IDs
        References (13)