FlyBase Allele Report: Dmel\lncRNA:DREAMer[KO]

General Information

Symbol

Dmel\lncRNA:DREAMer^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0322637

Feature type

allele

Associated gene

Dmel\lncRNA:DREAMer

Associated Insertion(s)

TI{TI}lncRNA:DREAMer^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Wen et al., 2016)

Mutagen

gene targeting by homologous recombination

(Wen et al., 2016)

CRISPR/Cas9

(Wen et al., 2016)

Progenitor genotype

Associated Insertion(s)

TI{TI}lncRNA:DREAMer^KO

Cytology

Description

The entire lncRNA gene has been knocked out and replaced with an attP site followed by an FRT-flanked Disc\RFP^3xP3.cUa fluorescent marker.

(Wen et al., 2016)

Allele components

Component

Use(s)

Also carries

attP

integrase target site

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:12,174,090..12,174,638 [-]

Reported_genomic_loc:

2L_r6:12174090..12174638

Comment:

lncRNA region removed by targeted deletion.

(Wen et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | larval stage

(Li et al., 2024)

increased size | larval stage (with Df(2L)ED775)

(Li et al., 2024)

increased size | larval stage | recessive

(Li et al., 2024)

male semi-fertile

(Wen et al., 2016)

viable

(Wen et al., 2016)

Phenotype Manifest In

embryonic/larval salivary gland | larval stage

(Li et al., 2024)

embryonic/larval salivary gland | larval stage (with Df(2L)ED775)

(Li et al., 2024)

nucleus | larval stage

(Li et al., 2024)

Detailed Description

Statement

Reference

CR43356^KO homozygote males show reduced fertility compared to wild-type controls.

(Wen et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

lncRNA:DREAMer^KO has increased size | recessive | larval stage phenotype, suppressible by E2f1ⁱ²/Scer\GAL4^ptc.PU

(Li et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

lncRNA:DREAMer^KO has embryonic/larval salivary gland | larval stage phenotype, suppressible by E2f1ⁱ²/Scer\GAL4^ptc.PU

(Li et al., 2024)

lncRNA:DREAMer^KO has nucleus | larval stage phenotype, suppressible by E2f1ⁱ²/Scer\GAL4^ptc.PU

(Li et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lncRNA:DREAMer^KO is rescued by lncRNA:DREAMer^UAS.RA/Scer\GAL4^Act.PU

(Li et al., 2024)

lncRNA:DREAMer^KO is rescued by Scer\GAL4^Act.PU/lncRNA:DREAMer^UAS.RB

(Li et al., 2024)

lncRNA:DREAMer^KO is rescued by Scer\GAL4^Act.PU/lncRNA:DREAMer^UAS.RC

(Li et al., 2024)

lncRNA:DREAMer^KO is rescued by lncRNA:DREAMer^gDNA

(Li et al., 2024)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CR43356^KO

DREAMer^KO

(Li et al., 2024)

lncRNA:CR43356^KO

lncRNA:DREAMer^KO

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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