FlyBase Allele Report: Dmel\Rh7[1]

General Information

Symbol

Dmel\Rh7¹

Species

D. melanogaster

Name

FlyBase ID

FBal0323541

Feature type

allele

Associated gene

Dmel\Rh7

Associated Insertion(s)

TI{TI}Rh7¹

Carried in Construct

Key Links

Allele class

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Ni et al., 2017)

Progenitor genotype

Associated Insertion(s)

TI{TI}Rh7¹

Cytology

Description

Deletion of 540bp immediately downstream of the Rh7 translation start site. A loxP cassette which contains the Scer\GAL4 coding region and a w^+mW.hs marker is inserted in place of the deleted sequence. The Scer\GAL4 gene is non-functional, due to a translation start site present in the upstream loxP site, which renders the Scer\GAL4 coding region out of frame.

(Ni et al., 2017)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage

(Ni et al., 2017)

abnormal circadian rhythm | adult stage

(Ni et al., 2017)

abnormal neurophysiology | adult stage

(Ni et al., 2017)

photoperiod response variant | adult stage

(Ni et al., 2017)

Phenotype Manifest In

l-LNv neuron | adult stage

(Ni et al., 2017)

Detailed Description

Statement

Reference

Rh7¹ homozygous adults previously trained under 12h:12h light:dark cycles fail to completely become arrhythmic under constant light conditions, present a severe decrease in circadian phase shifting in response to light pulses during the night period, present a significant delay in circadian phase shifting in response to a 8h delay in the light:dark transition, and present defective arousal in response to a light pulse during the night period - i.e. a significant decrease in the frequency of arousal and a significant delay in arousal -, but maintain a rhythmic behavior under constant darkness conditions and do not exhibit significant differences in the retinal response to light in electro-retinograms, as compared to controls; the large ventrolateral neurons (l-LNv) of the adult brain in these mutants exhibit a severe decrease in the frequency of firing in response to white (400-1000nm), blue (405nm) or orange light (550-1000nm), as compared to controls.

(Ni et al., 2017)

Temperature preferences of Rh7¹ homozygous mutant third instar larvae are unchanged compared to wild-type controls.

(Sokabe et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rh7¹ has photoperiod response variant | adult stage phenotype, enhanceable by cry⁰¹/cry⁰¹

(Ni et al., 2017)

Rh7¹ has photoperiod response variant | adult stage phenotype, enhanceable by gl^60j/gl^60j

(Ni et al., 2017)

Suppressed by

Statement

Reference

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Rh7^{+tCH322-180G19}

(Ni et al., 2017)

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Rh7^UAS.cNa/Scer\GAL4^P2.4.Pdf

(Ni et al., 2017)

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Scer\GAL4^tim.PE/Rh7^UAS.cNa

(Ni et al., 2017)

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Rh7^UAS.Tag:FLAG/Scer\GAL4^P2.4.Pdf

(Ni et al., 2017)

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Scer\GAL4^P2.4.Pdf/Rh3^UAS.cVa

(Ni et al., 2017)

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Rh4^UAS.cVa/Scer\GAL4^P2.4.Pdf

(Ni et al., 2017)

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype, suppressible | partially by Rh5^UAS.cVa/Scer\GAL4^P2.4.Pdf

(Ni et al., 2017)

Enhancer of

Statement

Reference

Rh7¹/Rh7¹ is an enhancer of photoperiod response variant | adult stage phenotype of cry⁰¹

(Ni et al., 2017)

Rh7¹/Rh7¹ is an enhancer of photoperiod response variant | adult stage phenotype of gl^60j

(Ni et al., 2017)

Other

Statement

Reference

Rh7¹, cry^b has abnormal circadian rhythm | adult stage phenotype

(Ni et al., 2017)

Rh7¹, gl^60j has abnormal circadian rhythm | adult stage phenotype

(Ni et al., 2017)

Rh7¹, cry⁰¹ has abnormal circadian rhythm | adult stage phenotype

(Ni et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Rh7¹, gl^60j double homozygous adults previously trained under 12h:12h light:dark cycles display both an increased proportion of arrhythmic individuals under constant darkness conditions and increased delay in circadian phase shifting to a 8h delay in the light:dark transition, as compared to either single homozygote.

Rh7¹, cry⁰¹ double homozygous adults exhibit an increased delay in circadian phase shifting to a 8h delay in the light:dark transition, as compared to either single homozygote. A significant proportion of Rh7¹/Rh7¹, cry⁰¹/cry⁰¹ adults, Rh7¹/Rh7¹, cry⁰¹/cry^b adults or Rh7¹/Rh7¹, cry⁰¹/cry^b adults, previously trained under 12h:12h light:dark cycles, fail to maintain circadian rhythmicity under constant darkness conditions, while the rhythmic individuals present a significantly longer period, as compared to controls. The defects of Rh7¹, cry^b double homozygotes to maintain rhythmicity under constant darkness is suppressed by the following conditions: the presence of Rh7^{+tCH322-180G19}; the expression of Rh7^Scer\UAS.cNa under the control of either Scer\GAL4^P2.4.Pdf or Scer\GAL4^tim.PE; the expression of Rh7^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^P2.4.Pdf; or the expression of Rh3^Scer.UAS.cVa, Rh4^Scer.UAS.cVa or Rh5^Scer.UAS.cVa under the control of Scer\GAL4^P2.4.Pdf.

(Ni et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rh7¹ is rescued by Rh7^{+tCH322-180G19}

(Ni et al., 2017)

Comments

Rh7¹ homozygous adults present a failure to become arrhythmic under constant darkness conditions and present a delay in circadian phase shifting to a 8h delay in the light:dark transition, both of which are rescued by the presence of Rh7^{+tCH322-180G19}.

(Ni et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76026

w¹¹¹⁸; P{CH322-180G19}attP40; TI{TI}Rh7¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rh7¹

(Piacenti-Silva et al., 2025, Meyerhof et al., 2024)

rh7¹

(Thakur et al., 2025, Leung et al., 2020, Li et al., 2020, Ni et al., 2017, Sokabe et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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