FlyBase Allele Report: Dmel\Mettl14[fs]

General Information

Symbol

Dmel\Mettl14^fs

Species

D. melanogaster

Name

FlyBase ID

FBal0324797

Feature type

allele

Associated gene

Dmel\Mettl14

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Lence et al., 2016)

Progenitor genotype

Cytology

Description

A 2-nucleotide frame shift at position 227 of the coding sequence, leading to a premature stop codon at amino acid position 89.

(Lence et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:8,308,842..8,308,847 [+]

Comment:

Replacement of CGACCT with TCCC leads to a -2 frameshift and early translation termination at aa89 after the addition of ~15 novel amino acids.

Inserted_sequence:

TCCC

(Lence et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with Df(2L)BSC200)

(Lence et al., 2016)

decreased efficacy of short-term memory | adult stage | progressive

(Kan et al., 2021)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mettl14^fs/Df(2L)BSC200 flies exhibit significant climbing defects, but do not show wing posture defects, as compared to controls.

(Lence et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Mettl14^fs/Df(2L)BSC200 has abnormal locomotor behavior phenotype, non-enhanceable by +/Df(3R)Exel6197

(Lence et al., 2016)

Enhancer of

Statement

Reference

Mettl14^fs/Df(2L)BSC200 is an enhancer of abnormal locomotor behavior phenotype of Mettl3^null/Df(3R)Exel6197

(Lence et al., 2016)

Other

Statement

Reference

Df(2L)BSC209/+, Mettl14^fs has partially lethal - majority live | female phenotype

(Knuckles et al., 2018)

Mettl14^fs/Df(2L)BSC200, Mettl3^null/Ime4[+] has visible phenotype

(Lence et al., 2016)

Phenotype Manifest In

Other

Statement

Reference

Mettl14^fs/Df(2L)BSC200, Mettl3^null/Ime4[+] has wing phenotype

(Lence et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2L)BSC209/+;Mettl14^fs/+ double heterozygous females show significantly reduced viability compared to Df(2L)BSC209 single heterozygotes.

(Knuckles et al., 2018)

Ime4^null/+, Mettl14^fs/Df(2L)BSC200 flies exhibit wing posture defects.

Df(3R)Exel6197/+ does not significantly worsen the climbing defects seen in Mettl14^fs/Df(2L)BSC200 flies.

Mettl14^fs/Df(2L)BSC200 significantly worsens the climbing defects seen in Ime4^null/Df(3R)Exel6197 flies.

(Lence et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

METTL14^fs

(Tang et al., 2021)

Mettl14^fs

(Kan et al., 2021, Worpenberg et al., 2021, Knuckles et al., 2018)

dMettl14fs

(Lence et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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