FlyBase Allele Report: Dmel\Mettl3[null]

General Information

Symbol

Dmel\Mettl3^null

Species

D. melanogaster

Name

FlyBase ID

FBal0324796

Feature type

allele

Associated gene

Dmel\Mettl3

Associated Insertion(s)

Carried in Construct

Also Known As

Mettl3 null

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lence et al., 2016)

Mutagen

CRISPR/Cas9

(Lence et al., 2016)

Progenitor genotype

Cytology

Description

A deletion of 1,291 bp (1,876-3,166 bp in the genome region chr3R:24030157..24034257 , genome assembly BDGP release 6), removing the entire coding sequence.

(Lence et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage (with Df(3R)Exel6197)

(Lence et al., 2016)

abnormal neuroanatomy | adult stage (with Mettl3^Δcat)

(Kan et al., 2021)

abnormal neuroanatomy | third instar larval stage

(Lence et al., 2016)

decreased efficacy of short-term memory | adult stage (with Mettl3^Δcat)

(Kan et al., 2021)

decreased efficacy of short-term memory | adult stage | progressive (with Df(3R)Exel6197)

(Kan et al., 2021)

partially lethal - majority die

(Rockwell and Hongay, 2020)

short lived (with Df(3R)Exel6197)

(Kan et al., 2021)

short lived (with Mettl3^Δcat)

(Kan et al., 2021)

viable

(Lence et al., 2016)

viable (with Mettl3^Δcat)

(Lence et al., 2016)

visible (with Mettl3^Δcat)

(Lence et al., 2016)

Phenotype Manifest In

medial lobe of adult mushroom body (with Mettl3^Δcat)

(Kan et al., 2021)

NMJ bouton | third instar larval stage

(Lence et al., 2016)

presynaptic active zone | third instar larval stage

(Lence et al., 2016)

wing (with Mettl3^Δcat)

(Lence et al., 2016)

Detailed Description

Statement

Reference

Ime4^null/Ime4^Δcat flies have a mild wing posture defect, as compared to controls.

Neuromuscular junction synapses in Ime4^null/Ime4^null mutants exhibit increased growth, as shown by an increased number of boutons and number of active zones per bouton, as compared to wild type. Ime4^null/+ and Ime4^null/Ime4^null flies do not show ovary encapsulation defects.

Ime4^null/Df(3R)Exel6197 flies exhibit significant climbing defects, as compared to controls.

(Lence et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Mettl3^null/Df(3R)Exel6197 has abnormal locomotor behavior phenotype, enhanceable by Mettl14^fs/Df(2L)BSC200

(Lence et al., 2016)

NOT suppressed by

Statement

Reference

Mettl3^null/Df(3R)Exel6197 has decreased efficacy of short-term memory | adult stage | progressive phenotype, non-suppressible by Ythdf^UAS.Tag:HA/Scer\GAL4^elav.PLu

(Kan et al., 2021)

Other

Statement

Reference

Df(2L)BSC209/+, Mettl3^null has partially lethal - majority die | female phenotype

(Knuckles et al., 2018)

Mettl14^fs/Df(2L)BSC200, Mettl3^null/Ime4[+] has visible phenotype

(Lence et al., 2016)

Mettl3^null, Sxl[+]/Sxl^fP7B0 has partially lethal - majority die | female limited phenotype

(Lence et al., 2016)

Phenotype Manifest In

Other

Statement

Reference

Mettl14^fs/Df(2L)BSC200, Mettl3^null/Ime4[+] has wing phenotype

(Lence et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Df(2L)BSC209/+;Mettl3^null/+ double heterozygous females show significantly reduced viability compared to Df(2L)BSC209 single heterozygotes.

(Knuckles et al., 2018)

Mettl14^fs/Df(2L)BSC200 significantly worsens the climbing defects seen in Ime4^null/Df(3R)Exel6197 flies.

Ime4^null/+, Sxl^fP7B0/+ females, but not males, exhibit severely reduced survival to hatching, as compared to controls.

Ime4^null/+, Mettl14^fs/Df(2L)BSC200 flies exhibit wing posture defects.

(Lence et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mettl3^Δcat/Mettl3^null is rescued by Mettl3^{UAS.cLa.Tag:HA}/Scer\GAL4^elav.PLu

(Kan et al., 2021)

Mettl3^Δcat/Mettl3^null is rescued by Scer\GAL4^Mef2.247/Mettl3^{UAS.cLa.Tag:HA}

(Kan et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Ime4^null

(Lence et al., 2016)

Ime4ΔNull

(Rockwell and Hongay, 2020)

METTL3^null

(Tang et al., 2021)

Mettl3 null

(Perlegos et al., 2022, Sami et al., 2022)

Mettl3^null

(Lancaster et al., 2024, Bawankar et al., 2021, Kan et al., 2021, Worpenberg et al., 2021, Knuckles et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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