FlyBase Allele Report: Dmel\kn[MI15480-TG4.2]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\kn^{MI15480-TG4.2}

General Information

Symbol

Dmel\kn^{MI15480-TG4.2}

Species

D. melanogaster

Name

FlyBase ID

FBal0325198

Feature type

allele

Associated gene

Associated Insertion(s)

Mi{Trojan-GAL4.2}kn^{MI15480-TG4.2}

Carried in Construct

Also Known As

Kn-Gal4

Key Links

Genomic Maps

Allele class

loss of function allele

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

loss of function allele

(Chao et al., 2017)

Mutagen

phiC31 integrase

(Gene Disruption Project members, 2016-)

Progenitor genotype

Mi{MIC}kn^MI15480

(Gene Disruption Project members, 2016-)

Associated Insertion(s)

Mi{Trojan-GAL4.2}kn^{MI15480-TG4.2}

Cytology

Description

The RMCE cassette of the Mi{MIC}kn^MI15480 insertion has been replaced with a Trojan-Gal4 cassette, thereby truncating the kn transcript and causing a severe loss-of-function or null allele.

(Chao et al., 2017)

The RMCE cassette of the Mi{MIC}kn^MI15480 insertion has been replaced with a Trojan-Gal4 cassette. The insertion is in a coding intron of kn and is predicted to gene trap the gene. (Note that if this gene has multiple transcripts, only those where the insertion is in a coding intron are predicted to be trapped).

(Gene Disruption Project members, 2016-)

Allele components

Component

Use(s)

Inserted element

Mi{Trojan-GAL4.2}

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:14,778,860..14,778,860 [-]

(Lee et al., 2018, Chao et al., 2017, Gene Disruption Project members, 2016-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2R)BSC429)

(Lee et al., 2018)

lethal (with kn^col-1)

(Chao et al., 2017)

lethal - all die during embryonic stage (with Df(2R)BSC429)

(Chao et al., 2017)

lethal - all die during embryonic stage | recessive

(Chao et al., 2017)

partially lethal - majority die (with kn¹)

(Chao et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(2R)BSC429/kn^{MI15480-TG4.2} has lethal - all die during embryonic stage phenotype, suppressible by Hsap\EBF3^{WT.UAS.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2}

(Chao et al., 2017)

NOT suppressed by

Statement

Reference

Df(2R)BSC429/kn^{MI15480-TG4.2} has lethal - all die during embryonic stage phenotype, non-suppressible by Hsap\EBF3^{Arg163Gln.UAS.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2}

(Chao et al., 2017)

Df(2R)BSC429/kn^{MI15480-TG4.2} has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^{kn-MI15480-TG4.2}/Hsap\EBF3^{Arg163Leu.UAS.Tag:HA}

(Chao et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(2R)BSC429/kn^{MI15480-TG4.2} is rescued by kn^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2}

(Lee et al., 2018)

Df(2R)BSC429/kn^{MI15480-TG4.2} is rescued by kn^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2}

(Chao et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

67516

y¹ w^*; Mi{Trojan-GAL4.2}kn^{MI15480-TG4.2}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

kn-T2A-Gal4

(Chao et al., 2017)

kn^{MI15480-TG4.2}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections