FlyBase Allele Report: Dmel\Opa1[K273A.UAS.Tag:HA]

General Information

Symbol

Dmel\Opa1^{K273A.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0325677

Feature type

allele

Associated gene

Dmel\Opa1

Associated Insertion(s)

Carried in Construct

P{UAS-Opa1.K273A.HA}

Key Links

Transgenic product class

missense_variant

(Tsuyama et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Tsuyama et al., 2017)

Mutagen

in vitro construct

(Tsuyama et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-Opa1.K273A.HA}

Cytology

Description

UASt regulatory sequences drive expression of Opa1 cDNA with a K273A amino acid substitution (essential for GTPase activity in human Opa1), tagged with 3x-Tag:HA.

(Tsuyama et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Opa1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.PF

(Nitta et al., 2024)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^smid-C161

(Tsuyama et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Opa1^{K273A.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 results in fragmented spherical mitochondrial morphology as well as shortening of the dendritic arbor in the class IV ddaC neurons in third instar larvae, moderate loss of dendrites is also observed in class III ddaA neurons (with the Scer\GAL4^smid-C161 driver) but not in the class I ddaE neurons (with the Scer\GAL4²²¹ driver).

(Tsuyama et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Opa1^{K273A.UAS.Tag:HA}, Scer\GAL4^ppk.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^109(2)80/PPP1R15^UAS.cMa

(Tsuyama et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Opa1^{K273A.UAS.Tag:HA}, Scer\GAL4^109(2)80 has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Opa1^{K273A.UAS.Tag:HA}, Scer\GAL4^109(2)80 has dendrite | third instar larval stage phenotype, suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of the dendritic arbor in class IV ddaC neurons observed in third instar larvae expressing Opa1^{K273A.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 can be rescued by co-expression of PPP1R15^Scer\UAS.cMa.

(Tsuyama et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Opa1^{K273A.Scer\UAS.T:Ivir\HA1}

Opa1^{K273A.UAS.Tag:HA}

Opa1[K273A]

(Tsuyama et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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