FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\AdukUAS.cBa
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General Information
Symbol
Dmel\AdukUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Braden
FlyBase ID
FBal0326150
Feature type
allele
Associated gene
Dmel\Aduk
Associated Insertion(s)
Carried in Construct
P{UAS-Aduk.B}
Key Links
Transgenic product class
cDNA
(Braden and Neufeld, 2016)
wild_type
(Braden and Neufeld, 2016)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Braden and Neufeld, 2016)
wild_type
(Braden and Neufeld, 2016)
Mutagen
in vitro construct
(Braden and Neufeld, 2016)
Progenitor genotype
Carried in construct
P{UAS-Aduk.B}
Cytology
Description

UASt regulatory sequences drive expression of the Aduk GM08204 cDNA clone.

(Braden and Neufeld, 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Aduk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of AdukScer\UAS.cBa under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C in fat body clones results in an increase in the number of autophagic vesicles (as detected by Atg8a) in well-fed larvae but does not perturb the appearance of autophageosomes in starved larvae relative to the neighboring cells. Scer\GAL4GMR.PU-driven expression leads to reduced adult eye size.

      (Braden and Neufeld, 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      AdukUAS.cBa, Scer\GAL4GMR.PU has visible | adult stage phenotype, non-enhanceable by Atg1GS10797, Scer\GAL4GMR.PU

      (Braden and Neufeld, 2016)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Clonal expression of AdukScer\UAS.cBa under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C in the fat body of Atg1Δ3D mutants leads to robust formation of Atg8a-positive (or LysoTracker positive) autophagosome vesicles in both fed and starved conditions, whereas no autophagy induction is observed in Atg13Δ81 mutant clones or clones co-expressing Atg1K38Q.Scer\UAS.

      The reduced eye size observed in adult flies expressing AdukScer\UAS.cBa under the control of Scer\GAL4GMR.PU is not further decreased by co-expression of Atg1GS10797 (although Atg1GS10797 expression on its own also leads to small eye phenotype).

      (Braden and Neufeld, 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      AdukScer\UAS.cBa
      AdukUAS.cBa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Braden
      Secondary FlyBase IDs
        References (1)