FB2026_03 , released September 17, 2026
Allele: Dmel\wash+NESΔNLS.GFP
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General Information
Symbol
Dmel\wash+NESΔNLS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0326193
Feature type
allele
Associated gene
Dmel\wash
Associated Insertion(s)
Carried in Construct
P{GFP-wash.+NESΔNLS}
Key Links
Transgenic product class
missense_variant
(Verboon et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Verboon et al., 2015)
Mutagen
in vitro construct
(Verboon et al., 2015)
Progenitor genotype
Carried in construct
P{GFP-wash.+NESΔNLS}
Cytology
Description

2.9kb genomic fragment that encompasses the entire wash gene. The wash nuclear localisation signal has been disrupted (WKRS to AAAA amino acid change). A strong nuclear export signal (LDELLELLRL) followed by a GFP tag has been added to the N-terminal end of the wash coding sequence.

(Verboon et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
wash
Encoded product / tool
wash
Tagged with
GFP
Tag:NES(Unk)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      wash+NESΔNLS.GFP fails to rescue washΔ185

      (Verboon et al., 2015)
      Comments

      wash+NESΔNLS.GFP fails tp rescue washΔ185, leading to similar nuclear phenotypes as washΔ185 alone (i.e. fails to rescues defects in nucleus shape, nucleus volume, Cajal body and nucleolus).

      (Verboon et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      wash+NESΔNLS.GFP
      wash+NESΔNLS.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)