FlyBase Allele Report: Dmel\wash[Δ185]

General Information

Symbol

Dmel\wash^Δ185

Species

D. melanogaster

Name

FlyBase ID

FBal0219165

Feature type

allele

Associated gene

Dmel\wash

Associated Insertion(s)

Carried in Construct

Also Known As

wash¹⁸⁵

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Nagel et al., 2017)

Mutagen

P-element activity

(Linardopoulou et al., 2007)

Progenitor genotype

P{EPgy2}wash^EY15549

(Linardopoulou et al., 2007)

Cytology

Description

Imprecise excision of P{EPgy2}wash^EY15549 has deleted more than half of the coding region of wash and introduced two stop codons at positions 11 and 12.

(Linardopoulou et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:12,180,729..12,181,756 [+]

Comment:

An imprecise excision event leads to two stop codons at positions 11 and 12 due to an internal deletion of 1,029

nucleotides and the insertion of six nucleotides (ATGATG) at the junction.

(Linardopoulou et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

autolysosome | third instar larval stage | nutrition conditional

(Nagel et al., 2017)

autophagosome | third instar larval stage

(Nagel et al., 2017)

autophagosome | third instar larval stage | nutrition conditional

(Nagel et al., 2017)

Cajal body | larval stage

(Verboon et al., 2015)

chromosome | larval stage

(Verboon et al., 2015)

cortical actin cytoskeleton | oogenesis

(Verboon et al., 2018)

cortical actin cytoskeleton | oogenesis (with Df(2R)BSC699)

(Verboon et al., 2018)

egg chamber

(Verboon et al., 2018)

egg chamber (with Df(2R)BSC699)

(Verboon et al., 2018)

embryonic/larval plasmatocyte | prepupal stage

(Nagel et al., 2017)

embryonic/larval plasmatocyte | pupal stage

(Nagel et al., 2017)

embryonic/larval plasmatocyte | third instar larval stage

(Nagel et al., 2017)

embryonic/larval tracheal dorsal trunk

(Dong et al., 2013)

fat body | third instar larval stage

(Nagel et al., 2017)

fat body | third instar larval stage | nutrition conditional

(Nagel et al., 2017)

female germline ring canal

(Verboon et al., 2018)

female germline ring canal (with Df(2R)BSC699)

(Verboon et al., 2018)

focal adhesion | pupal stage

(Nagel et al., 2017)

lysosome | pupal stage

(Nagel et al., 2017)

lysosome | third instar larval stage

(Nagel et al., 2017)

lysosome | third instar larval stage | nutrition conditional

(Nagel et al., 2017)

nucleolus | larval stage

(Verboon et al., 2015)

nucleus | larval stage

(Verboon et al., 2015)

oocyte

(Verboon et al., 2018)

oocyte (with Df(2R)BSC699)

(Verboon et al., 2018)

salivary gland | larval stage

(Verboon et al., 2015)

Detailed Description

Statement

Reference

wash^Δ185 homozygotes or wash^Δ185/Df(2R)BSC699 exhibit disrupted actin organization at outer ring canals in stage 7-9 egg chambers, premature ooplasmic streaming in stage 7 oocytes and disrupted cortical actin organization in stage 7-9 oocytes when compared to controls.

(Verboon et al., 2018)

wash^Δ185 homozygotes and wash^Δ185/Df(2R)BSC699 transheterozygotes are viable.

wash^Δ185 homozygous females present normal oogenesis and fertility, as there are no obvious defects in ring canals, actin cables in nurse cells, border cell migration, morphology of egg chamber, morphology of laid eggs, or number of offspring, as compared to controls; wash^Δ185 homozygous adults present a mild decrease in lifespan under sugar-only diet, as compared to controls.

wash^Δ185 homozygous larval and pupal macrophages show defects in flattening and spreading, as shown by decreased cell spread area on uncoated glass surfaces; mutant pupal macrophages also present decreased cell spread areas on vitronectin-coated glass surfaces, associated with significantly shorter focal adhesions, but not on Concavalin A-coated surfaces, as compared to controls; mutant pupal macrophages present enlarged Vha55-marked lysosomes and defects in neutralizing lysosomal pH after phagocytosis, despite an apparently normal phagocytic uptake, as compared to controls; prepupal macrophages present a significant decrease in cell area and motility defects, as shown by significant decreases in migratory velocity and distance in unperturbed conditions, and decreased numbers of macrophages reaching lesions 30min, but not 5min, after lesion induction, as compared to controls. wash^Δ185 homozygous third instar larval fat body cells display increased lysosomes and autophagosomes under both normal and sugar-only diet, as compared to controls; under sugar-only diet, there is an increase in markers for active autolysosomes, and lysosomes and autophagosomes also present increased enlargement, as compared to wild-type controls.

(Nagel et al., 2017)

wash^Δ185 is a suppressor of both bw[VDE2]/In(2R)bw^VDe2 and bw[D]/Dp(?;2)bw^D position effect variegation and is an enhancer of centromeric and heterochromatic (e.g. w[m4]/In(1)w^m4) position effect variegation, but has not effect on telomeric position effect variegation. The wash^Δ185 mutant larval salivary glands present the following severe nuclear defects, despite apparently normal gland and gland cell shapes, as compared to controls: irregular shape, with a wrinkled nuclear envelope; significant decrease in volume; significant decrease in DNA content; chromosomes positioned irregularly and closer to the nuclear periphery; and Cajal body and nucleolus defects (as shown by the mis-localization of the markers Coilin and Fibrillarin).

(Verboon et al., 2015)

Average dorsal trunk tube length is increased in stage 16 homozygous embryos compared to wild type.

(Dong et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

wash^Δ185 has egg chamber phenotype, non-enhanceable by SCAR^HMS01536/Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185 has cortical actin cytoskeleton | oogenesis phenotype, non-enhanceable by SCAR^HMS01536/Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185 has female germline ring canal phenotype, non-enhanceable by SCAR^HMS01536/Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

NOT suppressed by

Statement

Reference

wash^Δ185 has egg chamber phenotype, non-suppressible by SCAR^HMS01536/Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185 has cortical actin cytoskeleton | oogenesis phenotype, non-suppressible by SCAR^HMS01536/Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185 has female germline ring canal phenotype, non-suppressible by SCAR^HMS01536/Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

NOT Enhancer of

Statement

Reference

wash^Δ185/wash^Δ185 is a non-enhancer of cortical actin cytoskeleton | oogenesis phenotype of SCAR^HMS01536, Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185/wash^Δ185 is a non-enhancer of egg chamber phenotype of SCAR^HMS01536, Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185/wash^Δ185 is a non-enhancer of female germline ring canal phenotype of SCAR^HMS01536, Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

NOT Suppressor of

Statement

Reference

wash^Δ185/wash^Δ185 is a non-suppressor of cortical actin cytoskeleton | oogenesis phenotype of SCAR^HMS01536, Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185/wash^Δ185 is a non-suppressor of egg chamber phenotype of SCAR^HMS01536, Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

wash^Δ185/wash^Δ185 is a non-suppressor of female germline ring canal phenotype of SCAR^HMS01536, Scer\GAL4^{VP16.mat.αTub67C}

(Verboon et al., 2018)

Other

Statement

Reference

Polr2B^wimp, wash^Δ185 has oocyte phenotype

(Verboon et al., 2018)

Polr2B^wimp, wash^Δ185 has egg chamber phenotype

(Verboon et al., 2018)

Polr2B^wimp, wash^Δ185 has female germline ring canal phenotype

(Verboon et al., 2018)

Polr2B^wimp, wash^Δ185 has cortical actin cytoskeleton | oogenesis phenotype

(Verboon et al., 2018)

Polr2B^wimp, wash[+]/wash^Δ185 has nurse cell phenotype

(Liu et al., 2009)

Polr2B^wimp, wash[+]/wash^Δ185 has oocyte nucleus phenotype

(Liu et al., 2009)

Arpc1^Q25st, RpII140[+]/Polr2B^wimp, wash^Δ185 has nurse cell phenotype

(Liu et al., 2009)

Polr2B^wimp, wash[+]/wash^Δ185 has oocyte | oogenesis stage S7 phenotype

(Liu et al., 2009)

Rho1^1B, wash^Δ185 has oocyte | oogenesis stage S7 phenotype

(Liu et al., 2009)

capu^EY12344, wash^Δ185 has oocyte | oogenesis stage S7 phenotype

(Liu et al., 2009)

Arpc1^Q25st, wash^Δ185 has nurse cell phenotype

(Liu et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

wash^Δ185/+ RpII140^wimp/+ trans-heterozygotes exhibit disrupted actin organization at outer ring canals in stage 7-9 egg chambers, premature ooplasmic streaming in stage 7 oocytes and disrupted cortical actin organization in stage 7-9 oocytes when compared to controls.

(Verboon et al., 2018)

Stage 7 oocytes show premature cytoplasmic streaming in wash^Δ185/+ ; RpII140^wimp/+ females. The yolk granules show speeds of movement that are almost twice that seen in wild type and the movements are concerted and coordinated, in contrast to the uncoordinated, saltatory movements seen in wild-type oocytes at this stage. The normal gradient of microtubule organisation that is seen in wild-type oocytes at this stage is lost in the mutant oocytes. The oocyte nucleus is mislocalised in the mutant oocyte by stage 7 and contains to be mislocalised during later stages. The mutant stage 7 oocytes have a disrupted and discontinuous band of cortical actin.

Oocytes of wash^Δ185 Rho1^1B and wash^Δ185 capu^EY12344 transheterozygous females undergo premature cytoplasmic streaming. The normal gradient of microtubule organisation that is seen in wild-type oocytes at stage 7 is lost in the mutant oocytes. The mutant stage 7 oocytes have disruptions in the cortical actin.

wash^Δ185/+ ; RpII140^wimp/+ egg chambers show a loss of nurse cell integrity at stage 10a and incomplete stress fiber formation during during nurse cell dumping (stages 10b-11) compared to wild type. The actin network around the ring canals is abnormally expanded during nurse cell dumping in the mutant egg chambers. Inner ring canal formation appears to be unaffected, although ring canals separated from the nurse cell membrane can be found floating within the oocyte.

wash^Δ185 Arpc1^Q25st egg chambers show a loss of nurse cell integrity at stage 10a and incomplete stress fiber formation during during nurse cell dumping (stages 10b-11) compared to wild type. The actin network around the ring canals is abnormal by stage 10b.

wash^Δ185/+ ; RpII140^wimp/+ Arpc1^Q25st egg chambers show a loss of nurse cell integrity at stage 10a and incomplete stress fiber formation during during nurse cell dumping (stages 10b-11) compared to wild type. The actin network around the ring canals is abnormal by stage 10b.

(Liu et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

wash^Δ185 is rescued by wash^GFP

(Verboon et al., 2015)

Partially rescued by

wash^Δ185 is partially rescued by Scer\GAL4^Hml.Δ/wash^UAS.EGFP

(Nagel et al., 2017)

Not rescued by

wash^Δ185 is not rescued by wash^{+NESΔNLS.GFP}

(Verboon et al., 2015)

Comments

The expression of wash^{Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^Hml.Δ rescues the increased mortality under sugar-only diet and partially rescues the decreased macrophage cell spread area on uncoated surfaces, observed in wash^Δ185 homozygotes.

(Nagel et al., 2017)

wash^GFP, but not wash^{+NESΔNLS.GFP}, rescues wash^Δ185 to viability and rescues their nuclear defects (i.e. rescues defects in nucleus shape, nucleus volume, Cajal body and nucleolus).

(Verboon et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

79220

w¹¹¹⁸; wash^Δ185

28285

w¹¹¹⁸; wash^Δ185/CyO

Notes on Origin

Discoverer

The wash^Δ185 chromosome originally carried a separable mutation which was responsible for the lethality of the chromosome.

(Nagel et al., 2017)

Separable from an unrelated background lethal mutation, referred to as the 'not Wash lethal'.

(Verboon et al., 2018)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Wash^185*

(Green et al., 2018)

wash¹⁸⁵

(Strutt et al., 2019, Tsarouhas et al., 2019)

wash^Δ185

(Yamamoto-Hino et al., 2024, Davidson et al., 2023, Verboon et al., 2020, Verboon et al., 2018, Verboon et al., 2015, Rudrapatna et al., 2014, Dong et al., 2013, Liu et al., 2009, Linardopoulou et al., 2007)

washΔ185

(Nagel et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (13)

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