Ca-α1T^Gal4 homozygous adults previously trained under 12h:12h light:dark cycles show: a significant increase in sleep time during the light period, a significant increase in sleep bout length, and a significant increase in waking activity, regardless of being kept under the same 12h:12h light:dark cycles or being kept under constant darkness, as compared to controls; a significant decrease in sleep bout number when kept under the same 12h:12h light:dark cycles, but not when kept under constant darkness, as compared to controls; a small but significant increase in circadian period and a significant decrease in the proportion of individuals exhibiting circadian rhythmicity after being transferred into constant darkness conditions, as compared to controls; an insignificant increase in sleep recovery after 24h of mechanical sleep deprivation, as compared to controls.

Ca-α1T^Gal4 heterozygous adults expressing Ca-α1T^GD3252 under the control of Scer\GAL4^Ca-α1T (the intrinsic Gal4 driver of Ca-α1T^Gal4), previously trained under 12h:12h light:dark cycles, exhibit a significant decrease in sleep time when kept under the same light:dark cycles, but exhibit a significant increase in sleep time after being kept for 48h-96h under constant darkness, as compared to controls.