The first coding exon and flanking introns of Ca-α1T are replaced by a cassette containing a splice acceptor site, the GAL4 driver coding sequence and poly A termination sequence. This results in the GAL4 driver being expressed under the control of the endogenous Ca-α1T promoter. Since the inserted sequence includes a termination sequence, it is expected to produce a null Ca-α1T allele.
Ca-α1TGal4 drives expression in neurons labelled by Ca-α1TGFP.