FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Scer\GAL4gce-T2A-GAL4
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General Information
Symbol
Scer\GAL4gce-T2A-GAL4
Species
S. cerevisiae
Name
FlyBase ID
FBal0328040
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
TI{2A-GAL4}gceT2A-GAL4
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
gene targeting by homologous recombination
(Baumann et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{2A-GAL4}gceT2A-GAL4
Cytology
Description

The T2A viral peptide followed by the GAL4 open reading frame has been inserted in frame into the endogenous gce locus, just upstream of the stop codon. The presence of the T2A peptide is designed to ensure that the GAL4 driver sequence is translated independently and not as a fusion protein with the upstream coding sequence of the endogenous gce locus.

(Baumann et al., 2017)
Allele components
Component
Use(s)
Inserted element
TI{2A-GAL4}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      early third instar larval stage -- wandering third instar larval stage
      embryonic/larval corpus allatum
      (Baumann et al., 2017)
      embryonic/larval corpus cardiacum
      (Baumann et al., 2017)
      prothoracic gland
      (Baumann et al., 2017)
      wandering third instar larval stage
      embryonic/larval epidermis
      (Baumann et al., 2017)
      histoblast
      (Baumann et al., 2017)
      embryonic/larval oenocyte
      (Baumann et al., 2017)
      larval somatic muscle cell
      (Baumann et al., 2017)
      embryonic/larval mushroom body | intense
      (Baumann et al., 2017)
      embryonic/larval CNS subperineurial glial cell
      (Baumann et al., 2017)
      larval PTTH neuron of pars lateralis
      (Baumann et al., 2017)
      larval corpus allatum innervating neuron CA-LP 1
      (Baumann et al., 2017)
      larval corpus allatum innervating neuron CA-LP 2
      (Baumann et al., 2017)
      adult stage
      adult brain | restricted
      (Baumann et al., 2017)
      adult mushroom body | intense
      (Baumann et al., 2017)
      peptidergic neuron | subset
      (Baumann et al., 2017)
      adult central complex
      (Baumann et al., 2017)
      nodulus 3
      (Baumann et al., 2017)
      nodulus 2 | faint
      (Baumann et al., 2017)
      nodulus 1
      (Baumann et al., 2017)
      lobula | restricted
      (Baumann et al., 2017)
      medulla | restricted
      (Baumann et al., 2017)
      fan-shaped body layer 1
      (Baumann et al., 2017)
      fan-shaped body layer 2 -- fan-shaped body layer 3 | faint
      (Baumann et al., 2017)
      fan-shaped body layer 4
      (Baumann et al., 2017)
      fan-shaped body layer 5 | faint
      (Baumann et al., 2017)
      fan-shaped body layer 6 -- fan-shaped body layer 8
      (Baumann et al., 2017)
      ellipsoid body | intense
      (Baumann et al., 2017)
      second optic chiasma
      (Baumann et al., 2017)
      Additional Information
      Statement
      Reference

      In adults, ScerGAL4gce-T2A-GAL4 is expressed across the brain. Strong expression seen in the mushroom body and weak expression in nodulus subunit II compared to the other subunits. Expression is absent from T5 cells/lobula layer 1 and the lobula plate as well as Medulla layer M10.

      (Baumann et al., 2017)
       
      Marker for
      Reflects expression of
      gce
      (Baumann et al., 2017)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4gce-T2A-GAL4
      Name Synonyms
      Secondary FlyBase IDs
        References (1)