FlyBase Allele Report: Hsap\RELA[AD.R37G12]

General Information

Symbol

Hsap\RELA^AD.R37G12

Species

H. sapiens

Name

FlyBase ID

FBal0328781

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R37G12-p65.AD}

PBac{R37G12-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R37G12-p65.AD}

PBac{R37G12-p65.AD}

Cytology

Description

The GMR37G12 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR37G12

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult protocerebral bridge 1 glomerulus-fan-shaped body-round body type a neuron | subset

Comment: when combined with P{VT036267-GAL4.DBD.X} (combination referred to as 'P-Fr-GAL4')

(Xie et al., 2019)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Inactivation of P-Fr neutrons by the expression of Ctet\tetX^TNT-LC.UAS under the control of P-Fr split-Gal4 (Hsap\RELA^AD.R37G12 with Scer\GAL4^{DBD.VT036267.PX}) leads to resistance to sleep deprivation while it does not lead to any changes in the sleep/wake cycles, total duration of night time sleep, locomotion or mechanosensory responsiveness of flies when compared to control adults.

Thermogenetic activation of P-Fr neurons via expression of TrpA1^UAS.(B).cKa under the control of P-Fr split-Gal4 (Hsap\RELA^AD.R37G12 with Scer\GAL4^{DBD.VT036267.PX}) leads to significantly less sleep at night time, and a significant increase in locomotion of flies during nighttime with no change during daytime when compared to control adults.

(Xie et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Hsap\RELA^AD.R37G12, Scer\GAL4^{DBD.VT036267.PX}, TrpA1^UAS.(B).cKa has abnormal locomotor behavior | adult stage | heat sensitive phenotype

(Xie et al., 2019)

Hsap\RELA^AD.R37G12, Scer\GAL4^{DBD.VT036267.PX}, TrpA1^UAS.(B).cKa has abnormal sleep | adult stage | heat sensitive phenotype