FlyBase Allele Report: Scer\GAL4[DBD.R95H02]

General Information

Symbol

Scer\GAL4^DBD.R95H02

Species

S. cerevisiae

Name

FlyBase ID

FBal0332662

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R95H02-GAL4.DBD}

Also Known As

R95H02

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R95H02-GAL4.DBD}

Cytology

Description

The GMR95H02 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR95H02

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body dopaminergic neuron h1

Comment: when combined with P{R76F05-p65.AD} (combination referred to as 'SS01696')

(Truman et al., 2023, Saumweber et al., 2018)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | larval stage | conditional, with Crei\ChR2^{XXL.UAS.tdTomato}, Hsap\RELA^AD.R76F05

(Saumweber et al., 2018)

abnormal memory | larval stage | conditional, with Crei\ChR2^XXL.UAS, Hsap\RELA^AD.R76F05

(Saumweber et al., 2018)

abnormal memory | larval stage | nutrition conditional, with Hsap\KCNJ2^5XUAS.EGFP, Hsap\RELA^AD.R76F05

(Saumweber et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R95H02 and Hsap\RELA^AD.R76F05 in third instar larvae significantly impairs short-term associative appetitive olfactory memory following training with fructose, but not low salt or aspartic acid, with no effect on innate odor (n-amylacetate) or fructose preference, compared to controls.

Optogenetic activation (using Crei\ChR2^XXL.UAS or Crei\ChR2^{XXL.UAS.tdTomato} with 470 nm (blue) light, but not using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 630 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R95H02 and Hsap\RELA^AD.R76F05 in third instar larvae, in combination with a paired odor, results in a significant increase in short-term associative attractive olfactory memory, compared to controls.

(Saumweber et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions