FlyBase Allele Report: Hsap\RELA[AD.R76F05]

General Information

Symbol

Hsap\RELA^AD.R76F05

Species

H. sapiens

Name

FlyBase ID

FBal0304530

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R76F05-p65.AD}

PBac{R76F05-p65.AD}

Also Known As

R76F05

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - coding region fusion

(Aso et al., 2014)

in vitro construct - regulatory fusion

(Aso et al., 2014)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{R76F05-p65.AD}

PBac{R76F05-p65.AD}

Cytology

Nature of the lesion

Statement

Reference

The R76F05 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Product class / Tool use(s)

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Associated Sequence Features

GMR76F05

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval dopaminergic pPAM1 neuron

Comment: when combined with P{R95H02-GAL4.DBD} (combination referred to as 'SS01696')

(Saumweber et al., 2018)

adult stage

dopaminergic PAM neuron 2 | intense

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 4 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 6 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 3 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 10

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 5 | intense

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Yamagata et al., 2015)

dopaminergic PAM neuron 6 | intense

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Yamagata et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 2

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Aso and Rubin, 2016, Lewis et al., 2015)

dopaminergic PAM neuron 5

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Lewis et al., 2015, Aso et al., 2014)

dopaminergic PAM neuron 6

Comment: when combined with P{R80G12-GAL4.DBD} (combination referred to as 'MB056B')

(Lewis et al., 2015)

dopaminergic PAM neuron 1 | faint

Comment: when combined with P{R23C12-GAL4.DBD} (combination referred to as 'MB109B')

(Aso et al., 2014)

dopaminergic PAM neuron 11 | faint

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso et al., 2014)

dopaminergic PAM neuron 4

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso et al., 2014)

mushroom body beta lobe slice 1

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso and Rubin, 2016)

mushroom body beta lobe slice 2

Comment: when combined with P{R32G08-GAL4.DBD} (combination referred to as 'MB213B')

(Aso and Rubin, 2016)

dopaminergic PAL neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)

dopaminergic PAM neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)

dopaminergic PPL1 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)

dopaminergic PPM1 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)

dopaminergic PPM2 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)

dopaminergic PPM3 neuron | subset

Comment: when combined with P{Ddc-GAL4.DBD}

when combined with P{ple-GAL4.DBD}

(Cichewicz et al., 2017)

dopaminergic PPL2ab neuron | subset

Comment: when combined with P{ple-GAL4.DBD}

when combined with P{Ddc-GAL4.DBD}

(Cichewicz et al., 2017)

AOTU neuron of the dopaminergic PAL cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R61H03-GAL4.DBD}

(Xie et al., 2018)

SMP-gamma neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-D}

(Xie et al., 2018)

VMNP-LO neuron of the dopaminergic PAL cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-C}

(Xie et al., 2018)

dFB neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R60F07-GAL4.DBD}

(Xie et al., 2018)

mushroom body pedunculus-medial lobe arborizing neuron 1

Comment: when combined with P{DAT-B-GAL4.DBD}

when combined with P{R60F07-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

SMP-PED neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R60F07-GAL4.DBD}

(Xie et al., 2018)

WED neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{R60F07-GAL4.DBD}

when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-C}

when combined with P{ple-GAL4.DBD.TH-D}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

LAL-SEG neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-C}

when combined with P{ple-GAL4.DBD.TH-D}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

VLP neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{R61H03-GAL4.DBD}

when combined with P{ple-GAL4.DBD.TH-D}

(Xie et al., 2018)

mushroom body medial-vertical lobe arborizing neuron 1

Comment: when combined with P{R61H03-GAL4.DBD}

(Xie et al., 2018)

VLP-AMMC neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{ple-GAL4.DBD.TH-C}

when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

AUM neuron of the dopaminergic GNG cluster

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

LH-CA neuron of the dopaminergic PPL2ab cluster

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

SMP-PRW neuron of the dopaminergic PAL cluster

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

VLP-SAD neuron of the dopaminergic PPM2 cluster

Comment: when combined with P{ple-GAL4.DBD.TH-F}

(Xie et al., 2018)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R23C12

(Lewis et al., 2015)

abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R80G12

(Lewis et al., 2015)

abnormal chemosensitive behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.R23C12, TrpA1^UAS.cUa

(Lewis et al., 2015)

abnormal chemosensitive behavior | adult stage | heat sensitive, with Scer\GAL4^DBD.R80G12, TrpA1^UAS.cUa

(Lewis et al., 2015)

abnormal memory | larval stage | conditional, with Crei\ChR2^{XXL.UAS.tdTomato}, Scer\GAL4^DBD.R95H02

(Saumweber et al., 2018)

abnormal memory | larval stage | conditional, with Crei\ChR2^XXL.UAS, Scer\GAL4^DBD.R95H02

(Saumweber et al., 2018)

abnormal memory | larval stage | nutrition conditional, with Hsap\KCNJ2^5XUAS.EGFP, Scer\GAL4^DBD.R95H02

(Saumweber et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R95H02 and Hsap\RELA^AD.R76F05 in third instar larvae significantly impairs short-term associative appetitive olfactory memory following training with fructose, but not low salt or aspartic acid, with no effect on innate odor (n-amylacetate) or fructose preference, compared to controls.

Optogenetic activation (using Crei\ChR2^XXL.UAS or Crei\ChR2^{XXL.UAS.tdTomato} with 470 nm (blue) light, but not using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 630 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R95H02 and Hsap\RELA^AD.R76F05 in third instar larvae, in combination with a paired odor, results in a significant increase in short-term associative attractive olfactory memory, compared to controls.

(Saumweber et al., 2018)

Thermogenetic activation (using TrpA1^UAS.cUa at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C12 and Hsap\RELA^AD.R76F05, or the split-GAL4 pair of Scer\GAL4^DBD.R80G12 and Hsap\RELA^AD.R76F05, significantly reduces carbon dioxide avoidance in starved adults, compared to controls; the effect is significantly smaller in fed flies. There is no reduction in carbon dioxide avoidance when Scer\GAL4^DBD.R23C12 and Hsap\RELA^AD.R76F05 neurons are activated in association with carbon dioxide exposure in advance of, and not during, preference testing.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R23C12 and Hsap\RELA^AD.R76F05, or the split-GAL4 pair of Scer\GAL4^DBD.R80G12 and Hsap\RELA^AD.R76F05, in adults results in an attraction to the illuminated area, which is significant in the case of Scer\GAL4^DBD.R23C12 and Hsap\RELA^AD.R76F05 neurons, no attraction is observed in controls.

(Lewis et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

71136

w¹¹¹⁸; P{R76F05-p65.AD}attP40

68261

w¹¹¹⁸; P{R76F05-p65.AD}attP40; P{R23C12-GAL4.DBD}attP2

68276

w¹¹¹⁸; P{R76F05-p65.AD}attP40; P{R80G12-GAL4.DBD}attP2

68273

w¹¹¹⁸; P{R76F05-p65.AD}attP40; P{R32G08-GAL4.DBD}attP2/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

(Aso et al., 2014)

FlyBase curator comment: According to correspondence with the authors, some split combinations listed in the methods of the paper are incorrect; the correct combinations (MB002B=R12C11-AD+R14C08-DBD, MB316B=R58E02-AD+R93G08-DBD, MB056B=R76F05-AD+R80G12-DBD, MB047B=R58E02-AD+R93A09-DBD) are listed on FlyLight so these are the ones that have been associated with the paper.

(Lewis et al., 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\RELA^{AD.R76F05.T:Zzzz\Zip+}

Hsap\RELA^AD.R76F05

Name Synonyms

Secondary FlyBase IDs

References (16)

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