FlyBase Allele Report: Dmel\αTub84B[K40R]

General Information

Symbol

Dmel\αTub84B^K40R

Species

D. melanogaster

Name

FlyBase ID

FBal0336355

Feature type

allele

Associated gene

Dmel\αTub84B

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Mao et al., 2017)

gene targeting by homologous recombination

(Mao et al., 2017)

Progenitor genotype

Cytology

Description

Amino acid replacement: K40R.

(Mao et al., 2017)

Site-directed mutation (K40R) of the αTub84B locus, to mimic the non-acetylated protein.

(Mao et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:7,087,349..7,087,349 [+]

Nucleotide change:

A7087349G

Reported nucleotide change:

A?G

Amino acid change:

K40R | alphaTub84B-PA

Reported amino acid change:

K40R

(Mao et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Mao et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal touch perception | larval stage

(Yan et al., 2018)

abnormal touch response | larval stage

(Yan et al., 2018)

fertile

(Mao et al., 2017)

viable

(Mao et al., 2017)

Phenotype Manifest In

cytoplasmic microtubule | third instar larval stage | conditional

(Mao et al., 2017)

larval somatic muscle cell | third instar larval stage | conditional

(Mao et al., 2017)

Detailed Description

Statement

Reference

αTub84B^K40R mutant larvae show a significantly decreased response to gentle touch, as compared to controls.

(Yan et al., 2018)

αTub84B^K40R mutant third instar larvae do not display any apparent differences in the microtubule network in muscle cells compared to controls at room temperature but their microtubules (MT) are more sensitive to cold-induced destabilization as the perinuclear MTs are much less dense in αTub84B^K40R mutant muscle cells than in wild-type after cold treatment.

(Mao et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

αTub84B^K40R/αTub84B^K40R is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^elav.PU

(Mao et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

αTub84B^K40R/αTub84B^K40R is a suppressor | partially of larval somatic muscle cell | third instar larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^C57

(Mao et al., 2017)

αTub84B^K40R/αTub84B^K40R is a suppressor | partially of cytoplasmic microtubule | third instar larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^C57

(Mao et al., 2017)

αTub84B^K40R/αTub84B^K40R is a suppressor of NMJ bouton | increased number | third instar larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^elav.PU

(Mao et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The decreased density of perinuclear microtubules (MTs) in larval muscle cells characteristic for third instar larvae expressing Hsap\MAPT^{V337M.Scer\UAS} (with the Scer\GAL4^C57 driver) as well as the excess of satellite boutons at larval neuromuscular junctions (with the Scer\GAL4^elav.PU driver) is partially or fully suppressed (respectively) by αTub84B^K40R homozygosity.

(Mao et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

602673

w¹¹¹⁸; αTub84B^K40R

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

tub^K40R

(Niu et al., 2023)

α1-tubulin^K40R

(Niu et al., 2023)

αTub84B^K40R

(Yan et al., 2018)

αtub^K40R

(Mao et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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