FlyBase Allele Report: Dmel\Nlg4[UAS.cZa]

General Information

Symbol

Dmel\Nlg4^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zhang

FlyBase ID

FBal0336931

Feature type

allele

Associated gene

Dmel\Nlg4

Associated Insertion(s)

Carried in Construct

P{UAS-Nlg4.Z}

Key Links

Transgenic product class

cDNA

(Zhang et al., 2017)

wild_type

(Zhang et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhang et al., 2017)

wild_type

(Zhang et al., 2017)

Mutagen

in vitro construct

(Zhang et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-Nlg4.Z}

Cytology

Description

UASt regulatory sequences drive expression of the full-length Nlg4 GH07829 cDNA clone.

(Zhang et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nlg4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

(Zhang et al., 2017)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Zhang et al., 2017)

Phenotype Manifest In

NMJ bouton | increased number | third instar larval stage, with Scer\GAL4^elav.PU

(Zhang et al., 2017)

NMJ bouton | increased number | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Zhang et al., 2017)

Detailed Description

Statement

Reference

Expression of two copies of Nlg4^Scer\UAS.cZa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^RapGAP1-OK6 (but not Scer\GAL4^C57) leads to increased number of boutons as well as satellite boutons on neuromuscular junctions in third instar larvae, whereas expression of just one copy under the same drivers has no significant effect on bouton number.

(Zhang et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nlg4^UAS.cZa, Scer\GAL4^elav.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by tkv⁷/tkv[+]

(Zhang et al., 2017)

Nlg4^UAS.cZa, Scer\GAL4^elav.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by wit^A12/wit[+]

(Zhang et al., 2017)

Nlg4^UAS.cZa, Scer\GAL4^elav.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Mad^8-2/Mad[+]

(Zhang et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nlg4^UAS.cZa, Scer\GAL4^RapGAP1-OK6 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by tkv⁷/tkv[+]

(Zhang et al., 2017)

Nlg4^UAS.cZa, Scer\GAL4^RapGAP1-OK6 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by wit^A12/wit[+]

(Zhang et al., 2017)

Nlg4^UAS.cZa, Scer\GAL4^RapGAP1-OK6 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Mad^8-2/Mad[+]

(Zhang et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased bouton and satellite bouton number characteristic for third instar larvae expressing two copies of Nlg4^Scer\UAS.cZa under the control of Scer\GAL4^RapGAP1-OK6 is suppressed by combination with a single copy of tkv⁷ or wit^A12 or Mad^8-2.

(Zhang et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav.PU/Nlg4^UAS.cZa rescues Nlg4^KO10

(Zhang et al., 2017)

Scer\GAL4^RapGAP1-OK6/Nlg4^UAS.cZa rescues Nlg4^KO10

(Zhang et al., 2017)

Fails to rescue

Scer\GAL4^C57/Nlg4^UAS.cZa fails to rescue Nlg4^KO10

(Zhang et al., 2017)

Comments

The decreased number but increased size of boutons on neuromuscular junctions in Nlg4^KO10 mutant third instar larvae is fully rescued by expression of Nlg4^Scer\UAS.cZa if driven by either Scer\GAL4^elav.PU or Scer\GAL4^RapGAP1-OK6 but not Scer\GAL4^C57. Similarly, the functional neurotransmission defects (reduced quantal content, decreased amplitude of evoked excitatory junction potentials) along with the larval locomotor deficit are rescued by Scer\GAL4^elav.PU-driven expression of Nlg4^Scer\UAS.cZa but not if it is controlled by Scer\GAL4^C57.

(Zhang et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nlg4^Scer\UAS.cZa

Nlg4^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zhang

Secondary FlyBase IDs

References (1)

Report Sections

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