FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Ecol\lexAbnl-lexA
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General Information
Symbol
Ecol\lexAbnl-lexA
Species
E. coli
Name
FlyBase ID
FBal0337957
Feature type
allele
Associated gene
Ecol\lexA
Associated Insertion(s)
TI{2A-lexA::p65}bnllexA
Carried in Construct
Also Known As
bnl-LexA
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Du et al., 2017)
ends-out gene targeting
(Du et al., 2017)
Progenitor genotype
Associated Insertion(s)
TI{2A-lexA::p65}bnllexA
Cytology
Description

A region in the middle of the first coding bnl exon has been replaced by sequence that encodes a lexA::p65 driver. A T2A self-cleaving peptide is present upstream of the driver sequence and a stop codon is present downstream of the driver sequence, to ensure that the driver is translated independently and not as part of a fusion protein with bnl. This results in the expression of a lexA::p65 driver under the control of the endogenous bnl regulatory sequences, including 5' and 3' UTR sequence, while the presence of the stop codon is expected to disrupt bnl function.

(Du et al., 2017)
Allele components
Component
Use(s)
Inserted element
TI{2A-lexA::p65}
Encoded product / tool
lexA::p65
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      air sac primordium
      (Du et al., 2017)
      embryonic/larval tracheal transverse connective 5
      (Du et al., 2017)
      embryonic/larval tracheal transverse connective 4
      (Du et al., 2017)
      embryonic/larval dorsal tracheal branch 2
      (Du et al., 2017)
      male genital disc | restricted
      (Du et al., 2017)
      wing disc | restricted
      (Du et al., 2017)
      Additional Information
      Statement
      Reference

      Ecol\lexAbnl-lexA drives expression in a pattern consistent with that of endogenous bnl at embryonic and larval stages.

      (Du et al., 2017)
       
      Marker for
      Reflects expression of
      bnl
      (Du et al., 2017)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lexAbnl-lexA
      bnllexA
      (Blackie et al., 2024)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)