FlyBase Allele Report: Dmel\Pi3K92E[UAS.cUa]

General Information

Symbol

Dmel\Pi3K92E^UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0340212

Feature type

allele

Associated gene

Dmel\Pi3K92E

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K92E.U}

Also Known As

UAS-Dp110

Key Links

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Pi3K92E.U}

Cytology

Description

UAS regulatory sequences drive expression of Pi3K92E.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pi3K92E

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of high grade glioma

CEA

(Witte et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates high grade glioma

modeled by btl::Egfr^λ.UAS

(Witte et al., 2009)

model of high grade glioma

is exacerbated by btl::Egfr^λ.UAS

(Witte et al., 2009)

exacerbates cancer

modeled by Myc^UAS.cZa, p53^{RNAi.UAS.RasG12D}

(Diaz et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

autolysosome | third instar larval stage, with Scer\GAL4^Act5C.PP

(Wu et al., 2009)

Bolwig nerve, with Scer\GAL4^repo

(Witte et al., 2009)

cell body, with Scer\GAL4^smid-C161

(Brierley et al., 2009)

cell body | somatic clone, with Scer\GAL4^VGlut1-OK371

(Brierley et al., 2009)

dendrite, with Scer\GAL4^smid-C161

(Brierley et al., 2009)

dendrite | somatic clone, with Scer\GAL4^VGlut1-OK371

(Brierley et al., 2009)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Act5C.PP

(Wu et al., 2009)

embryonic/larval gut | third instar larval stage, with Scer\GAL4^Act5C.PP

(Wu et al., 2009)

embryonic/larval optic stalk, with Scer\GAL4^repo

(Witte et al., 2009)

eye disc | larval stage, with Scer\GAL4^repo

(Witte et al., 2009)

gastric caecum | somatic clone, with Scer\GAL4^Act5C.PP

(Denton et al., 2012)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^smid-C161

(Brierley et al., 2009)

larval glial cell, with Scer\GAL4^repo

(Witte et al., 2009)

motor neuron | somatic clone, with Scer\GAL4^VGlut1-OK371

(Brierley et al., 2009)

neuropil | somatic clone, with Scer\GAL4^VGlut1-OK371

(Brierley et al., 2009)

Detailed Description

Statement

Reference

Mosaic clones overexpressing Pi3K92E^Scer\UAS.cWa in the gastric caeca of the midgut are enlarged and show reduced autophagy compared to surrounding wild type cells.

(Denton et al., 2012)

Scer\GAL4^smid-C161-mediated expression of Pi3K92E^Scer\UAS.cWa in the class IV dendritic aborizing sensory neuron ddaC results in a two-fold increase in mean soma volume and dendritic branch number.

Single cell leg motoneuron clones expressing Pi3K92E^Scer\UAS.cWa induced 96 hr after hatching have the following phenotypes: a two-fold increase in mean soma volume that is mirrored by an increase in the complexity of dendritic branching; the mean centre of mass is the same as controls; there is no major shift of dendrites to the midline. In a dorsoventral plane, these clones have a more complex dendritic pattern but cover much the same territory as wild type clones.

(Brierley et al., 2009)

Expression of Pi3K92E^UAS.cUa in larvae under the control of Scer\GAL4^repo results in a thickened optic stalk due to increased numbers of glial cells; there is also overmigration of glial cells along the Bolwig nerve and glial cell numbers are significantly reduced in the eye imaginal disc at later stages of eye disc development, compared to controls.

(Witte et al., 2009)

Clones of larval intestinal epithelial cells or fat body cells expressing Pi3K92E^Scer\UAS.cWa are refractory to the induction of autophagy seen in wild type clones upon starvation.

(Wu et al., 2009)

Expression of Pi3K92E^Scer\UAS.cWa under the control of Scer\GAL4^elav-C155 results in a significant reduction in diapause compared with control flies.

Expression of Pi3K92E^Scer\UAS.cWa in the visual system, under the control of Scer\GAL4^GMR.PF does not significantly affect diapause.

(Williams et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pi3K92E^UAS.cUa, Scer\GAL4^repo has abnormal cell migration | larval stage phenotype, enhanceable by btl::Egfr^λ.UAS, Scer\GAL4^repo

(Witte et al., 2009)

Enhancer of

Statement

Reference

Pi3K92E^UAS.cUa, Scer\GAL4^repo is an enhancer of abnormal cell migration | larval stage phenotype of Scer\GAL4^repo, btl::Egfr^λ.UAS

(Witte et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pi3K92E^UAS.cUa, Scer\GAL4^repo has Bolwig nerve phenotype, enhanceable by btl::Egfr^λ.UAS, Scer\GAL4^repo

(Witte et al., 2009)

Suppressed by

Statement

Reference

Pi3K92E^UAS.cUa, Scer\GAL4^repo has eye disc | larval stage phenotype, suppressible by btl::Egfr^λ.UAS, Scer\GAL4^repo

(Witte et al., 2009)

Enhancer of

Statement

Reference

Pi3K92E^UAS.cUa, Scer\GAL4^repo is an enhancer of Bolwig nerve phenotype of Scer\GAL4^repo, btl::Egfr^λ.UAS

(Witte et al., 2009)

Suppressor of

Statement

Reference

Pi3K92E^UAS.cUa, Scer\GAL4^repo is a suppressor of eye disc | larval stage phenotype of Scer\GAL4^repo, btl::Egfr^λ.UAS

(Witte et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Addition of btl::Egfr^λ.UAS to larvae expressing Pi3K92E^UAS.cUa under the control of Scer\GAL4^repo, suppresses the phenotype of decreased glial cells in the eye disc and enhances the phenotype of increased glial migration along the Bolwig nerve.

(Witte et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pi3K92E^Scer\UAS.cUa

Pi3K92E^Scer\UAS.cWa

Pi3K92E^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

FBal0327042
FBal0240564

References (11)

Report Sections

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