Stage 16 en::hth^{1-430.Scer\UAS}; Scer\GAL4^ato.3.6 embryos have a high percentage of abnormally oriented lch5 chordotonal organs. These abnormally oriented lch5 organs appear to be fully differentiated but lack ligament attachment cells. In fact, no ligament attachment cells are present in segments that exhibit abnormally oriented lch5 chordotonal organs.

At 29^oC, expression of en::hth^{1-430.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 results in pupal lethality. At 24^oC, pupal development and metamorphosis are completed but pharate adults are unable to emerge from the pupal case. The eyes and eye imaginal discs are often enlarged and ectopic eyes are occasionally seen on the ventral head capsule and antennae. There are often numerous ectopic bristles at different locations along the eye margin. All flies show a partial antenna to leg transformation; the first and second segments appear normal and the third segment is normal proximally, but the distal portion is transformed into leg. The transformed structure is rounded and densely covered by bracted bristles, typical of distal leg, and mostly resembles a tibia. The endogenous legs are also severely malformed and truncated. Proximal leg structures (coxa and trochanter) are reduced in size and fused together. More distal leg structures consist of a round mass, which appears to be a shortened, thickened femur fused to an extremely thickened and rounded tibia. Small segments with the appearance of distal tarsus or pretarsus are present in some legs, but more distal structures (such as the claws) are not present. Leg discs lose their normal structure of concentric folds and appear bloated. Wing hinge structures are replaced by abnormal tissue in flies expressing en::hth^{1-430.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 and bracted bristles are seen in this region. The anterior region of the wing blade is distorted, having the same cuticular appearance as the wing hinge. Bristles of the anterior wing margin are condensed into a very small areas. The posterior part of the wing blade differentiates into wing blade tissue but does not have wing veins. A duplication of the wing is seen in some cases, with the ectopic wing extending from the mesothorax, dorsal to the original wing and smaller in size. The wing disc is substantially enlarged, the folds of the blade region do not form properly and large epithelial sacs protrude from the normal plane of the disc. Outgrowths are occasionally seen extending bilaterally from the labellum in flies expressing en::hth^{1-430.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. The outgrowths have bracted bristles, suggesting a distal leg identity.

hth^{1-430.EnR.UAS}/Scer\GAL4^ey.PH is a suppressor of eye phenotype of L²

hth^{1-430.EnR.UAS}/Scer\GAL4^ey.PH is a suppressor of eye disc phenotype of L²