FlyBase Allele Report: Dmel\drpr[indel]

General Information

Symbol

Dmel\drpr^indel

Species

D. melanogaster

Name

FlyBase ID

FBal0343393

Feature type

allele

Associated gene

Dmel\drpr

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Sapar et al., 2018)

Progenitor genotype

Cytology

Description

Insertion of 2 nucleotides (CT) after the T in the 9th codon, resulting in a frame shift. The next in-frame Met codon is after the signal peptide, thus it is predicted that any translated protein should not be properly processed and targeted to the plasma membrane.

(Sapar et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion

3L:1,724,067..1,724,068 [-]

Inserted_sequence:

Comment:

Insertion of two bases (CT) after the T in the ninth codon of drpr resulting in a frameshift.

(Sapar et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Sung et al., 2024, Ji et al., 2022)

abnormal neuroanatomy | P-stage

(Sung et al., 2024)

abnormal stress response | larval stage

(Ji et al., 2022)

increased size | larval stage

(Ji et al., 2022)

Phenotype Manifest In

dendrite | larval stage

(Sung et al., 2024, Ji et al., 2022)

dendrite | P-stage

(Sung et al., 2024)

larval dorsal multidendritic neuron ddaC

(Ji et al., 2022)

larval dorsal multidendritic neuron ddaC | larval stage

(Sung et al., 2024)

larval dorsal multidendritic neuron ddaC | P-stage

(Sung et al., 2024)

Detailed Description

Statement

Reference

drpr^indel homozygous larvae do not show significant dendrite debris from class IV dendritic arborizing neurons on the underlying epidermal cells.

(Sapar et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

drpr^indel has abnormal stress response | larval stage phenotype, enhanceable by Spyo\Cas9^SOP.scE1x8/axed^U6:3.gRNA

(Ji et al., 2022)

NOT Enhanced by

Statement

Reference

drpr^indel has abnormal neuroanatomy | P-stage phenotype, non-enhanceable by ctg^wgd/galectin^wgv

(Sung et al., 2024)

Suppressed by

Statement

Reference

drpr^indel has abnormal neuroanatomy | larval stage phenotype, suppressible by Spyo\Cas9^ppk.PP/Nmnat^U6:3.gRNA

(Ji et al., 2022)

drpr^indel has increased size | larval stage phenotype, suppressible by Spyo\Cas9^ppk.PP/Nmnat^U6:3.gRNA

(Ji et al., 2022)

NOT suppressed by

Statement

Reference

drpr^indel has abnormal neuroanatomy | P-stage phenotype, non-suppressible by ctg^wgd/galectin^wgv

(Sung et al., 2024)

Enhancer of

Statement

Reference

drpr^indel/drpr^indel is an enhancer of abnormal stress response | larval stage phenotype of Spyo\Cas9^SOP.scE1x8, axed^U6:3.gRNA

(Ji et al., 2022)

Suppressor of

Statement

Reference

drpr^indel/drpr^indel is a suppressor of abnormal neuroanatomy | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of decreased size | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of abnormal neuroanatomy | first instar larval stage | progressive phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of abnormal neuroanatomy | second instar larval stage | progressive phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of abnormal size | larval stage phenotype of Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2}, Cdc50^sgRNA.U6, Scer\GAL4^DcG, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

NOT Suppressor of

Statement

Reference

drpr^indel/drpr^indel is a non-suppressor of abnormal neuroanatomy | third instar larval stage | progressive phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

drpr^indel has dendrite | larval stage phenotype, enhanceable by Spyo\Cas9^SOP.scE1x8/axed^U6:3.gRNA

(Ji et al., 2022)

drpr^indel has larval dorsal multidendritic neuron ddaC | conditional phenotype, enhanceable by Spyo\Cas9^SOP.scE1x8/axed^U6:3.gRNA

(Ji et al., 2022)

NOT Enhanced by

Statement

Reference

drpr^indel has larval dorsal multidendritic neuron ddaC | P-stage phenotype, non-enhanceable by ctg^wgd/galectin^wgv

(Sung et al., 2024)

drpr^indel has dendrite | P-stage phenotype, non-enhanceable by ctg^wgd/galectin^wgv

(Sung et al., 2024)

Suppressed by

Statement

Reference

drpr^indel has dendrite | larval stage phenotype, suppressible by Spyo\Cas9^ppk.PP/Nmnat^U6:3.gRNA

(Ji et al., 2022)

drpr^indel has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Spyo\Cas9^ppk.PP/Nmnat^U6:3.gRNA

(Ji et al., 2022)

NOT suppressed by

Statement

Reference

drpr^indel has larval dorsal multidendritic neuron ddaC | P-stage phenotype, non-suppressible by ctg^wgd/galectin^wgv

(Sung et al., 2024)

drpr^indel has dendrite | P-stage phenotype, non-suppressible by ctg^wgd/galectin^wgv

(Sung et al., 2024)

Enhancer of

Statement

Reference

drpr^indel/drpr^indel is an enhancer of dendrite | larval stage phenotype of Spyo\Cas9^SOP.scE1x8, axed^U6:3.gRNA

(Ji et al., 2022)

drpr^indel/drpr^indel is an enhancer of larval dorsal multidendritic neuron ddaC | larval stage phenotype of Spyo\Cas9^SOP.scE1x8, axed^U6:3.gRNA

(Ji et al., 2022)

Suppressor of

Statement

Reference

drpr^indel/drpr^indel is a suppressor of dendrite | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of dendrite | first instar larval stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of larval dorsal multidendritic neuron ddaC | first instar larval stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of dendrite | second instar larval stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of larval dorsal multidendritic neuron ddaC | second instar larval stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

drpr^indel/drpr^indel is a suppressor of larval multidendritic class IV neuron | larval stage phenotype of Cdc50^sgRNA.U6, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

drpr^indel/drpr^indel is a suppressor of larval multidendritic class IV neuron | larval stage phenotype of Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2}, Cdc50^sgRNA.U6, Scer\GAL4^DcG, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

drpr^indel/drpr^indel is a suppressor of larval multidendritic class IV neuron | larval stage phenotype of Cdc50^sgRNA.U6, Ecol\lexA^ppk.PU, Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG}, Spyo\Cas9^ppk.PS

(Sapar et al., 2018)

NOT Suppressor of

Statement

Reference

drpr^indel/drpr^indel is a non-suppressor of dendrite | third instar larval stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

drpr^indel/drpr^indel is a non-suppressor of larval dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^ppk.PH

(Ji et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The combined tissue-specific knockout of CDC50 (CDC50^sgRNA.U6+Spyo\Cas9^ppk.PS) and expression of Mmus\Ano6^{D430G-L.lexAop.Tag:FLAG} under the control of Ecol\lexA^ppk.PU leads to significant vesicle shedding by class IV dendritic arborizing neurons compared to controls, which is suppressed by drpr^indel homozygosity.

The combined tissue-specific knockout of CDC50 (CDC50^sgRNA.U6+Spyo\Cas9^ppk.PS) and expression of Avic\GFP^{sfGFP.UAS.Tag:mLactC1C2} under the control of Scer\GAL4^DcG leads to short dendrites in class IV dendritic arborizing neurons, as compared to CDC50 knockout alone; this phenotype is suppressed by drpr^indel homozygosity.

(Sapar et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

drpr^indel

(Sung et al., 2024, Ji et al., 2022, Sapar et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (4)

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