FlyBase Allele Report: Dmel\Elys[5]

General Information

Symbol

Dmel\Elys⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0343419

Feature type

allele

Associated gene

Dmel\Elys

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

loss of function allele

(Hirai et al., 2018)

Mutagen

CRISPR/Cas9

(Hirai et al., 2018)

Progenitor genotype

Cytology

Description

Deletion of 3476 nucleotides.

(Sawamura, 2019.1.25)

Deletion plus 8bp insertion within the coding sequence. This results in a frameshift.

(Hirai et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

X:19,653,587..19,657,061 [+]

Inserted_sequence:

CTCGGTCG

Comment:

Deletion of Elys residues X:19653587..19657061 and insertion of 8 bases (CTCGGTCG) at the same site leads to a frameshift at codon 335 and a premature translation stop after the addition of 13 novel amino acids.

(Sawamura, 2019.1.25)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | embryonic stage | maternal effect

(Hirai et al., 2025)

decreased occurrence of cell division | embryonic cycle 1 | recessive

(Hirai et al., 2018)

female fertile (with Dp(1;3)DC365)

(Hirai et al., 2018)

female sterile (with Df(1)BSC871)

(Hirai et al., 2018)

female sterile (with Df(1)ED7620)

(Hirai et al., 2018)

female sterile (with Elys²)

(Hirai et al., 2018)

female sterile | recessive

(Hirai et al., 2018)

lethal | maternal effect

(Hirai et al., 2018)

lethal | maternal effect (with Df(1)BSC871)

(Hirai et al., 2018)

lethal | maternal effect (with Df(1)ED7620)

(Hirai et al., 2018)

lethal | maternal effect (with Elys²)

(Hirai et al., 2018)

lethal - all die during embryonic stage | maternal effect

(Hirai et al., 2025)

male fertile

(Hirai et al., 2018)

viable

(Hirai et al., 2018)

Phenotype Manifest In

centrosome | embryonic cycle 1

(Hirai et al., 2018)

mitotic spindle | embryonic cycle 1

(Hirai et al., 2018)

Detailed Description

Statement

Reference

The progeny of Elys⁵ homozygous females (and wild-type males) arrest at prometaphase/metaphase of the first cleavage division; free asters are observed in the embryo, the microtubule arrays in the spindle conjugate indiscriminately with chromosomes, sister centrosomes separate, one-to-all four centrosome may detach from the spindle pole; polar bodies anomalously form bipolar spindles that lack centrosomes. Eggs laid by Elys⁵ homozygous females are successfully penetrated by sperm of control individuals.

(Hirai et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Elys⁵ has increased mortality during development | maternal effect phenotype, suppressible | partially by Scer\GAL4^nanos.PG/Dsim\Elys^UASp.cHa

(Hirai et al., 2025)

Elys⁵ has increased mortality during development | maternal effect phenotype, suppressible | partially by Scer\GAL4^nanos.PG/Dsim\Elys^UASp.mCherry