FlyBase Allele Report: Dmel\mir-285[KO.w-]

General Information

Symbol

Dmel\mir-285^KO.w-

Species

D. melanogaster

Name

FlyBase ID

FBal0343834

Feature type

allele

Associated gene

Dmel\mir-285

Associated Insertion(s)

TI{TI}mir-285^KO.w-

Carried in Construct

Key Links

Allele class

Mutagen

cre recombinase

Nature of the Allele

Allele class

Mutagen

cre recombinase

(Li et al., 2017)

Progenitor genotype

TI{TI}mir-285^KO

(Li et al., 2017)

Associated Insertion(s)

TI{TI}mir-285^KO.w-

Cytology

Description

The loxP cassette containing the w^+mW.hs marker has been excised from TI{TI}mir-285^KO.

(Li et al., 2017)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | larval stage

(Li et al., 2017)

fertile

(Li et al., 2017)

viable

(Li et al., 2017)

Phenotype Manifest In

embryonic/larval CNS subperineurial glial cell | larval stage

(Li et al., 2017)

larval brain | larval stage

(Li et al., 2017)

nucleus | larval stage

(Li et al., 2017)

perineurium | adult stage

(Li et al., 2017)

perineurium | adult stage (with Df(3L)BSC458)

(Li et al., 2017)

perineurium | adult stage (with Df(3L)Exel6115)

(Li et al., 2017)

perineurium | larval stage

(Li et al., 2017)

perineurium | larval stage (with Df(3L)BSC458)

(Li et al., 2017)

perineurium | larval stage (with Df(3L)Exel6115)

(Li et al., 2017)

septate junction | larval stage

(Li et al., 2017)

Detailed Description

Statement

Reference

mir-285^KO.w- homozygotes are viable, fertile, and show no obvious developmental defect when raised under normal conditions.

mir-285^KO.w-/mir-285^KO.w-, mir-285^KO.w-/Df(3L)Exel6115 and mir-285^KO.w-/Df(3L)BSC458 adults and larvae have a compromised blood-brain barrier, as dye injected into the body cavity is able to diffuse into their adult retina or third instar larval brain; this is not observed in mir-285^KO.w-/Df(3L)BSC840 or in mir-285^KO.w-/+ and wild-type controls. Subperineurial glial cells in mir-285^KO.w-/mir-285^KO.w- larvae frequently show disrupted/discontinuous septate junctions (Dlg immunostaining), and exhibit significant increases in nuclear size and in ploidy, as compared to controls. mir-285^KO.w-/mir-285^KO.w- larvae also exhibit a significant increase in brain volume.