GAL4::VP16 and a Disc\RFP3xP3.cUa marker flanked by loxP sites is inserted in Lk immediately following the ATG translational start site (+4bp to +10bp, relative to start site).
LkUAS.cZa/Scer\GAL4Lk-GAL4::VP16 partially rescues LkGAL4::VP16